| Author | Song, Hao | |
| Author | Zhao, Zhennan | |
| Author | Chai, Yan | |
| Author | Jin, Xiyue | |
| Author | Li, Changyao | |
| Author | Yuan, Fei | |
| Author | Liu, Sheng | |
| Author | Gao, Zhengrong | |
| Author | Wang, Haiyuan | |
| Author | Song, Jian | |
| Author | Vazquez, Leonardo | |
| Author | Zhang, Yanfang | |
| Author | Tan, Shuguang | |
| Author | Morel, Carlos M. | |
| Author | Yan, Jinghua | |
| Author | Shi, Yi | |
| Author | Qi, Jianxun | |
| Author | Gao, Feng | |
| Author | Gao, George F. | |
| Access date | 2023-07-03T22:44:54Z | |
| Available date | 2023-07-03T22:44:54Z | |
| Document date | 2019 | |
| Citation | SONG, Hao et al. Molecular Basis of Arthritogenic Alphavirus Receptor MXRA8 Binding to Chikungunya Virus Envelope Protein. Cell, v. 177, n. 7, p. 1714-1724, Jun. 2019. | en_US |
| ISSN | 0092-8674 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59386 | |
| Language | eng | en_US |
| Publisher | MIT Press | en_US |
| Rights | open access | en_US |
| MeSH | Animals | en_US |
| MeSH | Chikungunya virus | en_US |
| MeSH | Chlorocebus aethiops | en_US |
| MeSH | HEK293 Cells | en_US |
| MeSH | Humans | en_US |
| MeSH | Membrane Proteins | en_US |
| MeSH | Protein Domains | en_US |
| MeSH | Vero Cells | en_US |
| MeSH | Viral Envelope Proteins | en_US |
| MeSH | Virus Internalization | en_US |
| Title | Molecular Basis of Arthritogenic Alphavirus Receptor MXRA8 Binding to Chikungunya Virus Envelope Protein | en_US |
| Type | Article | en_US |
| DOI | 10.1016/J.CELL.2019.04.008 | |
| Abstract | Arthritogenic alphaviruses, such as Chikungunya virus (CHIKV), cause severe and debilitating rheumatic diseases worldwide, resulting in severe morbidity and economic costs. Recently, MXRA8 was reported as an entry receptor. Here, we present the crystal structures of the mouse MXRA8, human MXRA8 in complex with the CHIKV E protein, and the cryo-electron microscopy structure of human MXRA8 and CHIKV virus-like particle. MXRA8 has two Ig-like domains with unique structural topologies. This receptor binds in the "canyon" between two protomers of the E spike on the surface of the virion. The atomic details at the interface between the two binding entities reveal that both the two domains and the hinge region of MXRA8 are involved in interaction with CHIKV E1-E2 residues from two protomers. Notably, the stalk region of MXRA8 is critical for CHIKV virus entry. This finding provides important information regarding the development of therapeutic countermeasures against those arthritogenic alphaviruses. | en_US |
| Affilliation | Research Network of Immunity and Health. Beijing Institutes of Life Science. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China / Savaid Medical School. University of Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China / School of Life Sciences. University of Science and Technology of China. Hefei, China. | en_US |
| Affilliation | College of Veterinary Medicine. China Agricultural University. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China / School of Life Sciences. University of Science and Technology of China. Hefei, China. | en_US |
| Affilliation | Kunming Institute of Zoology. Chinese Academy of Sciences. Kunming, China. | en_US |
| Affilliation | College of Animal Sciences and Technology. Guangxi University. Nanning, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China / Oswaldo Cruz Foundation. Center for Technological Development in Health. National Institute of Science and Technology for Innovation on Diseases of Neglected Populations. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China. / Savaid Medical School. University of Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Center for Technological Development in Health. National Institute of Science and Technology for Innovation on Diseases of Neglected Populations. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China / Savaid Medical School. University of Chinese Academy of Sciences. Beijing, China / CAS Center for Influenza Research and Early-warning. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China / Savaid Medical School. University of Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | Tianjin Institute of Industrial Biotechnology. Chinese Academy of Sciences. Tianjin, China / Institute of Genetics and Developmental Biology. Chinese Academy of Sciences. Beijing, China. | en_US |
| Affilliation | Research Network of Immunity and Health. Beijing Institutes of Life Science. Chinese Academy of Sciences. Beijing, China / CAS Key Laboratory of Pathogenic Microbiology and Immunology. Institute of Microbiology. Chinese Academy of Sciences. Beijing, China / Savaid Medical School. University of Chinese Academy of Sciences. Beijing, China / School of Life Sciences. University of Science and Technology of China. Hefei, China / CAS Center for Influenza Research and Early-warning. Chinese Academy of Sciences. Beijing, China / Tianjin Institute of Industrial Biotechnology. Chinese Academy of Sciences. Tianjin, China / National Institute for Viral Disease Control and Prevention. Chinese Center for Disease Control and Prevention. Beijing, China. | en_US |
| Subject | Adhesion molecule | en_US |
| Subject | Arthritogenic alphavirus | en_US |
| Subject | Chikungunya virus | en_US |
| Subject | MXRA8 | en_US |
| Subject | Recepor binding | en_US |
| Subject | Virus entry | en_US |
| Subject | Human monoclonal-antibodies | en_US |
| Subject | Crystal-structure | en_US |
| Subject | Contact | en_US |
| Subject | Reveals | en_US |
| Subject | Dicam | en_US |
| Subject | Entry | en_US |
| Subject | HEK293 Cells | |
| e-ISSN | 1097-4172 | |
