| Author | Lage, Ramon Silva | |
| Author | Oliveira, Guilherme Corrêa de | |
| Author | Busek, Solange Cristina Uber | |
| Author | Guerra, Luanda Liboreiro | |
| Author | Giunchetti, Rodolfo Cordeiro | |
| Author | Oliveira, Rodrigo Corrêa | |
| Author | Reis, Alexandre Barbosa | |
| Access date | 2023-07-04T11:49:59Z | |
| Available date | 2023-07-04T11:49:59Z | |
| Document date | 2007 | |
| Citation | LAGE, Ramon Silva et al. Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi. Veterinary Immunology and Immunopathology, v. 115, n. 1-2, p. 135-145, 2007. | en_US |
| ISSN | 0165-2427 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59389 | |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | restricted access | |
| Title | Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi | en_US |
| Type | Article | |
| DOI | 10.1016/j.vetimm.2006.10.001 | |
| Abstract | Recent studies suggest that asymptomatic dogs infected with canine visceral leishmaniasis (CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-infected animals present a Th2 profile. In the present study, an RT-PCR method has been standardised and employed to evaluate the frequency and the semi-quantitative level of expression of the cytokines IL-4, IL- 10, IL- 12, INF-gamma and TNF-alpha in splenocytes of 30 dogs naturally infected with Leishmania chagasi and of 7 non-infected dogs (NID). An increase in the level of expression of IL-12 (p = 0.059) was detected in all CVL-infected dogs compared with NID. In dogs exhibiting high parasitism, the frequency of expression of IL-10 was higher (p = 0.011) than in animals presenting low parasitism or medium parasitism (MP) and in NID animals, whilst the level of expression of IL- 10 was higher (p = 0.0094) than in animals exhibiting MP and in the NID group. Positive correlations between the levels of expression of IL-10 with respect to the progression of the disease (IL-10: r = 0.3510; p = 0.0337) and the levels of expression of IL-10 and INF-gamma increase in parasitism (IL-10: r = 0.3428; p = 0.0438 and INF-gamma: r = 0.4690; p = 0.0045) were observed. Such data suggest that CVL is marked by a balanced production of Th1 and Th2 cytokines, with a predominant accumulation of IL-10 as a consequence of an increase in parasitic load and progression of the disease, and INF-gamma was related with the increase in parasitic load. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil / Santa Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil / Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brazil. | en_US |
| Subject | Cytokines | en_US |
| Subject | RT-PCR | en_US |
| Subject | Canine diseases | en_US |
| Subject | Visceral leishmaniasis | en_US |
| Subject | Leishmania chagasi | en_US |
| Embargo date | 2099-12-31 | |
