| Author | Oliveira, Débora L. | |
| Author | Fonseca, Fernanda L. | |
| Author | Zamith-Miranda, Daniel | |
| Author | Nimrichter, Leonardo | |
| Author | Rodrigues, Jéssica | |
| Author | Pereira, Marcos D. | |
| Author | Reuwsaat, Julia C. V. | |
| Author | Schrank, Augusto | |
| Author | Staats, Charley | |
| Author | Kmetzsch, Livia | |
| Author | Vainstein, Marilene H. | |
| Author | Rodrigues, Marcio L. | |
| Access date | 2023-07-18T06:50:32Z | |
| Available date | 2023-07-18T06:50:32Z | |
| Document date | 2016 | |
| Citation | OLIVEIRA, Débora L. et al. The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans. Future Microbiology, v. 11, n. 11, p. 1405-1405, 2016. | en_US |
| ISSN | 1746-0913 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59639 | |
| Language | eng | en_US |
| Publisher | Future Medicine | en_US |
| Rights | restricted access | |
| MeSH | Animals | en_US |
| MeSH | Arthropods | en_US |
| MeSH | Autophagy | en_US |
| MeSH | Autophagy-Related Protein 7 | en_US |
| MeSH | Cryptococcosis | en_US |
| MeSH | Cryptococcus neoformans | en_US |
| MeSH | DNA Fungal | en_US |
| MeSH | Disease Models Animal | en_US |
| MeSH | Female | en_US |
| MeSH | Fungal Proteins | en_US |
| MeSH | Gene Deletion | en_US |
| MeSH | Gene Expression Regulation Fungal | en_US |
| MeSH | Genes Fungal | en_US |
| MeSH | Larva | en_US |
| MeSH | Lung | en_US |
| MeSH | Mice | en_US |
| MeSH | Mice, Inbred C57BL | en_US |
| MeSH | Nitrogen | en_US |
| MeSH | Oxygen | en_US |
| MeSH | Phagocytes | en_US |
| MeSH | Pigments, Biological | en_US |
| MeSH | Sequence Deletion | en_US |
| MeSH | Survival | en_US |
| MeSH | Virulence | en_US |
| Title | The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans | en_US |
| Type | Article | |
| DOI | 10.2217/FMB-2016-0090 | |
| Abstract | AIM We investigated the involvement of the autophagy protein 7 (Atg7) in physiology and pathogenic potential of Cryptococcus neoformans. MATERIALS & METHODS The C. neoformans gene encoding Atg7 was deleted by biolistic transformation for characterization of autophagy mechanisms, pigment formation, cell dimensions, interaction with phagocytes and pathogenic potential in vivo. RESULTS & CONCLUSION ATG7 deletion resulted in defective autophagy mechanisms, enhanced pigmentation and increased cellular size both in vitro and in vivo. The atg7Δ mutant had decreased survival in the lung of infected mice, higher susceptibility to the killing machinery of different host phagocytes and reduced ability to kill an invertebrate host. These results connect Atg7 with mechanisms of pathogenicity in the C. neoformans model. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Bioquímica, Instituto de Química. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal Do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brazil. | en_US |
| Subject | ATG7 | en_US |
| Subject | Autophagy | en_US |
| Subject | Cryptococcus neoformans | en_US |
| Subject | Macrophages | en_US |
| Subject | Virulence | en_US |
| Subject | Autophagy-related protein 7 | en_US |
| Subject | Inbred c57bl | en_US |
| e-ISSN | 1746-0921 | |
| Embargo date | 2030-12-31 | |
