| Author | Giannini, Maria José Soares Mendes | |
| Author | Andreotti, Patrícia Ferrari | |
| Author | Vincenzi, Luciana Raquel | |
| Author | Silva, Juliana Leal Monteiro da | |
| Author | Lenzi, Henrique Leonel | |
| Author | Benard, Gil | |
| Author | Oliveira, Roseli Zancopé | |
| Author | Guedes, Herbert Leonel de Matos | |
| Author | Soares, Christiane Pienna | |
| Access date | 2012-12-12T16:01:18Z | |
| Available date | 2012-12-12T16:01:18Z | |
| Document date | 2006 | |
| Citation | ANDREOTTI, P. F. et. al. Binding of extracellular matrix proteins to Paracoccidioides brasiliensis. Microbes and Infection, v. 8, p. 1550-1559, 2006. | pt_BR |
| ISSN | 1286-4579 | |
| URI | https://www.arca.fiocruz.br/handle/icict/5967 | |
| Sponsorship | Acknowledgements
This investigation was financially supported by the Brazilian organizations, FAPESP, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and
PADC-FCF-UNESP. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | en |
| Rights | open access | |
| Subject in Portuguese | Clínica médica | pt_BR |
| Subject in Portuguese | Dermatopatias infecciosas | pt_BR |
| Subject in Portuguese | Paracoccidioidomicose | pt_BR |
| Subject in Portuguese | Matriz extracelular | pt_BR |
| Subject in Portuguese | Células epiteliais | pt_BR |
| Title | Binding of extracellular matrix proteins to Paracoccidioides brasiliensis | pt_BR |
| Type | Article | |
| DOI | http://dx.doi.org/10.1016/j.micinf.2006.01.012 | |
| Abstract | Adhesion to extracellular matrix (ECM) proteins plays a crucial role in invasive fungal diseases. ECM proteins bind to the surface of Paracoccidioides brasiliensis yeast cells in distinct qualitative patterns. Extracts from Pb18 strain, before (18a) and after animal inoculation (18b), exhibited differential adhesion to ECM components. Pb18b extract had a higher capacity for binding to ECM components than Pb18a. Laminin was the most adherent component for both samples, followed by type I collagen, fibronectin, and type IV collagen for Pb18b. A remarkable difference was seen in the interaction of the two extracts with fibronectin and their fragments. Pb18b extract interacted significantly with the 120-kDa fragment. Ligand affinity binding assays showed that type I collagen recognized two components (47 and 80 kDa) and gp43 bound both fibronectin and laminin. The peptide 1 (NLGRDAKRHL) from gp43, with several positively charged amino acids, contributed most to the adhesion of P. brasiliensis to Vero cells. Synthetic peptides derived from peptide YIGRS of laminin or from RGD of both laminin and fibronectin showed the greatest inhibition of adhesion of gp43 to Vero cells. In conclusion, this work provided new molecular details on the interaction between P. brasiliensis and ECM components. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Laboratório de Alergia e Imunologia Clínica e Experimental. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil. | pt_BR |
| Subject | Paracoccidioides brasiliensis | pt_BR |
| Subject | Extracellular matrix | pt_BR |
| Subject | Adhesins | pt_BR |
| Subject | gp43 | pt_BR |
| Subject | Células epiteliais | pt_BR |
| DeCS | Medicina Clínica | pt_BR |
| DeCS | Dermatopatias Infecciosas | pt_BR |
| DeCS | Paracoccidioidomicose | pt_BR |
| DeCS | Matriz Extracelular | pt_BR |
| DeCS | Adesinas Bacterianas | pt_BR |
