| Author | Azevedo, Vasco | |
| Author | Costa, Marcilia P | |
| Author | McCulloch, John A | |
| Author | Almeida, Síntia S | |
| Author | Dorella, Fernanda A | |
| Author | Fonseca, Cristina Toscano | |
| Author | Oliveira, Diana M | |
| Author | Teixeira, Maria FS | |
| Author | Laskowska, Ewa | |
| Author | Lipinska, Barbara | |
| Author | Meyer, Roberto | |
| Author | Portela, Ricardo W | |
| Author | Oliveira, Sérgio C | |
| Access date | 2023-07-19T19:00:49Z | |
| Available date | 2023-07-19T19:00:49Z | |
| Document date | 2011 | |
| Citation | COSTA, Marcilia P et al. Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice. BMC Res Notes, v. 4, 243, 2011. doi: 10.1186/1756-0500-4-243. | en_US |
| ISSN | 1756-0500 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59681 | |
| Language | eng | en_US |
| Publisher | Biomed Central | en_US |
| Rights | open access | en_US |
| Title | Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice | en_US |
| Type | Article | en_US |
| DOI | 10.1186/1756-0500-4-243 | |
| Abstract | Background: Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants.
Findings: To construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60).
Conclusion: This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil/Universidade Estadual do Ceará. Núcleo de Genômica e Bioinformática. Faculdade de Medicina Veterinária. Fortaleza, CE, Brazil | en_US |
| Affilliation | Universidade Federal do Pará, Instituto de Ciências Biológicas. Departamento de Genética. Belém, PA, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Estadual do Ceará. Núcleo de Genômica e Bioinformática. Faculdade de Medicina Veterinária. Fortaleza, CE, Brazil | en_US |
| Affilliation | Universidade Estadual do Ceará. Núcleo de Genômica e Bioinformática. Faculdade de Medicina Veterinária. Fortaleza, CE, Brazil | en_US |
| Affilliation | Department of Biochemistry. University of Gdansk, ul. Bażyńskiego. Gdańsk, Poland | en_US |
| Affilliation | Department of Biochemistry. University of Gdansk, ul. Bażyńskiego. Gdańsk, Poland | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brazil | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | en_US |
