Author | Lemos, Elenice Moreira | |
Author | Gomes, Izabelle Teixeira | |
Author | Carvalho, Sılvio Fernando Guimaraes | |
Author | Rocha, Roberta Dias Rodrigues | |
Author | Pissinate, Jauber Fornaciari | |
Author | Martins Filho, Olindo Assis | |
Author | Dietze, Reynaldo | |
Access date | 2023-07-20T12:39:27Z | |
Available date | 2023-07-20T12:39:27Z | |
Document date | 2007 | |
Citation | LEMOS, Elenice Moreira et al. Detection of anti-leishmania (Leishmania) chagasi immunoglobulin G by flow cytometry for cure assessment following chemotherapeutic treatment of American visceral leishmaniasis. Clin Vaccine Immunol., v. 14, n. 5, p. 569-576, 2007. doi: 10.1128/CVI.00354-06. | en_US |
ISSN | 1556-6811 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59692 | |
Sponsorship | CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO | en_US |
Language | eng | en_US |
Publisher | American Society for Microbiology | en_US |
Rights | restricted access | en_US |
Title | Detection of anti-Leishmania (Leishmania) chagasi immunoglobulin g by flow cytometry for cure assessment following chemotherapeutic treatment of American visceral leishmaniasis | en_US |
Type | Article | en_US |
DOI | 10.1128/CVI.00354-06 | |
Abstract | The residual serological reactivity observed in patients cured of visceral leishmaniasis (VL) represents the major factor underlying the low efficiency of most anti-Leishmania serological approaches to assess posttherapeutic cure in VL. Herein, we have described a detuned How cytometry-based methodology to detect anti-live (FC-ALPA-immunoglobulin G [IgG]) and anti-fixed (FC-AFPA-IgG) L. chagasi promastigote IgG, along the titration curve (1:2,000 to 1:128,000), as a tool to assess late (12 months after treatment [12 mAT]) and early (2 and 6 mAT) posttherapeutic cure of pediatric American visceral leishmaniasis. Reactivities were reported as the percentage of positive fluorescent parasite (PPFP), using a PPFP of 50% as a cutoff to segregate positive and negative results. Our data demonstrated that both FC-ALPA-IgG at 1:4,000 and FC-ALPA-IgG at 1:32,000 are useful for late cure assessment in VL, with 100% specificity and outstanding likelihood ratio indices. Cure assessment at 6 mAT also showed promising performance indices, identifying 81% and 71.4% of the treated patients with negative results. However, new interpretation parameters were necessary to monitor cure at 2 mAT. We then introduced the differential PPFP (Delta PPFP) of 25% as a new cutoff for early cure assessment at specific serum dilutions to analyze IgG reactivity by FC-ALPA-IgG and FC-AFPA-IgG. Our data demonstrated that at 2 mAT, Delta PPFP was > 25% in 60% and 57.1% of treated patients, whereas at 6 mAT, a Delta PPFP of > 25% was observed in 100% and 95.2% of samples assayed by FC-ALPA-IgG and FC-AFPA-IgG, respectively. Together, our findings showed the potential of both FC-ALPA-IgG and FC-AFPA-IgG regarding their applicability to detect differential serological reactivity and further contribution to posttherapeutic cure assessment in VL | en_US |
Affilliation | Nucleo de Doenças Infecciosas. Universidade Federal do Espırito Santo. Vitoria, ES, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Universidade Estadual de Montes Claros. Montes Claros, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Nucleo de Doenças Infecciosas. Universidade Federal do Espırito Santo. Vitoria, ES, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Nucleo de Doenças Infecciosas. Universidade Federal do Espırito Santo. Vitoria, ES, Brasil | en_US |
Embargo date | 2030-12-31 | |