| Author | Sanchez, Bruno Antonio Marinho | |
| Author | Varotti, Fernando de Pilla | |
| Author | Rodrigues, Flávia Guimarães | |
| Author | Carvalho, Luzia Helena | |
| Access date | 2023-07-21T14:19:16Z | |
| Available date | 2023-07-21T14:19:16Z | |
| Document date | 2007 | |
| Citation | SANCHEZ, Bruno Antonio Marinho et al. Validation of a Plasmodium falciparum parasite transformed with green fluorescent protein for antimalarial drug screening. J Microbiol Methods, v. 69, n. 3, p. 518-522, 2007. doi: 10.1016/j.mimet.2007.03.001. | en_US |
| ISSN | 0167-7012 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59709 | |
| Sponsorship | CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO | en_US |
| Language | eng | en_US |
| Publisher | Elsevier Biomedical | en_US |
| Rights | restricted access | |
| Title | Validation of a Plasmodium falciparum parasite transformed with green fluorescent protein for antimalarial drug screening | en_US |
| Type | Article | |
| DOI | 10.1016/j.mimet.2007.03.001 | |
| Abstract | Aiming to replace the radioisotopic assay, the widely used procedure for vitro antimalarial drug screening, we set up a protocol using a Plasmodium falciparum strain transformed with the green fluorescent protein (PfGFP), which can be quickly and specifically quantified by flow cytometry. On the basis of a side-by-side comparison, this PfGFP-based method showed results similar to those obtained with the standard radioisotopic method. (C) 2007 Elsevier B.V. All rights reserved | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | en_US |
| Subject | green fluorescent protein | en_US |
| Subject | Plasmodium falciparum | en_US |
| Subject | chemotherapy | en_US |
| Subject | In vitro | en_US |
| Embargo date | 2099-12-31 | |
