| Author | Simões, Mariana Crivellari Machado | |
| Author | Bahia, Diana | |
| Author | Zerlotini, Adhemar | |
| Author | Torres, Kleider | |
| Author | Artiguenave, François | |
| Author | Neshich, Goran | |
| Author | Kuser, Paula | |
| Author | Oliveira, Guilherme Correa de | |
| Access date | 2023-07-27T18:57:29Z | |
| Available date | 2023-07-27T18:57:29Z | |
| Document date | 2007 | |
| Citation | SIMÕES, Mariana Crivellari Machado et al. Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni. Mol Biochem Parasitol, v. 154, n. 2, p. 134–140, 2007. doi: 10.1016/j.molbiopara.2007.04.003 | en_US |
| ISSN | 0166-6851 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59815 | |
| Language | eng | en_US |
| Publisher | Elsevier/North-Holland Biomedical Press. | en_US |
| Rights | restricted access | |
| Title | Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni | en_US |
| Type | Article | |
| DOI | doi:10.1016/j.molbiopara.2007.04.003 | |
| Abstract | Single nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm 14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope. (c) 2007 Elsevier B.V. All rights reserved | en_US |
| Affilliation | Laboratory of Cellular and Molecular Parasitology. René Rachou Research Centre. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Laboratory of Cellular and Molecular Parasitology. René Rachou Research Centre. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Laboratory of Cellular and Molecular Parasitology. René Rachou Research Centre. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Laboratory of Cellular and Molecular Parasitology. René Rachou Research Centre. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | INRA-Unité de Recherche Genomique Info. Evry cedex, France. | en_US |
| Affilliation | Embrapa/Informática Agropecuária. Department of Structural Bioinformatics. Campinas, SP, Brazil. | en_US |
| Affilliation | Embrapa/Informática Agropecuária. Department of Structural Bioinformatics. Campinas, SP, Brazil. | en_US |
| Affilliation | Laboratory of Cellular and Molecular Parasitology. René Rachou Research Centre. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Santa Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Schistosoma mansoni | en_US |
| Subject | single nucleotide polymorphism | en_US |
| Subject | computational biology | en_US |
| Subject | cathepsin B | en_US |
| Subject | comparative modeling | en_US |
| Embargo date | 2099-12-31 | |
