| Author | Vilela, Márcia Carvalho | |
| Author | Mansur, Daniel Santos | |
| Author | Lacerda-Queiroz, Norinne | |
| Author | Rodrigues, David Henrique | |
| Author | Lima, Graciela Kunrath | |
| Author | Arantes, Rosa Maria Esteves | |
| Author | Kroon, Erna Geessien | |
| Author | Campos, Marco Antonio da Silva | |
| Author | Teixeira, Mauro Martins | |
| Author | Teixeira, Antonio Lucio | |
| Access date | 2023-07-31T12:48:10Z | |
| Available date | 2023-07-31T12:48:10Z | |
| Document date | 2009 | |
| Citation | VILELA, Márcia Carvalho et al. The chemokine CCL5 is essential for leukocyte recruitment in a model of severe Herpes simplex encephalitis. Ann N Y Acad Sci., v. 1153, p. 256-263. doi: 10.1111/j.1749-6632.2008.03959.x. | en_US |
| ISSN | 0077-8923 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59847 | |
| Language | eng | en_US |
| Publisher | Blackwell | en_US |
| Rights | restricted access | |
| Title | The Chemokine CCL5 Is Essential for Leukocyte Recruitment in a Model of Severe Herpes simplex Encephalitis | en_US |
| Type | Article | |
| DOI | 10.1111/j.1749-6632.2008.03959.x | |
| Abstract | The Herpes simplex virus-1 (HSV-1) is responsible for several clinical manifestations in humans, including encephalitis. To induce encephalitis, C57BL/6 mice were inoculated with 104 plaque-forming cells of HSV-1 by the intracranial route. Met-RANTES (regulated upon activation, normal T cell expressed and presumably secreted) (10 mu g/mouse), a CC chemokine family receptor (CCR)1 and CCR5 antagonist, was given subcutaneously the day before, immediately after, and at days 1, 2, and 3 after infection. Treatment with Met-RANTES had no effect on the viral titers. In contrast, intravital microscopy revealed that treatment with Met-RANTES decreased the number of leukocytes adherent to the pial microvasculature at days I and 3 after infection. The levels of the chemokines CCL3, CCL5, CXCL1, and CXCL9 increased after infection and were enhanced further by the treatment with Met-RANTES. Treatment with a polyclonal anti-CCL5 antibody 2 h before the intravital microscopy decreased leukocyte adhesion in the microcirculation of infected mice. In conclusion, CCL5, a chemokine that binds to CCR1 and CCR5, is essential for leukocyte adhesion during HSV-1 encephalitis. However, blocking of CCR1 and CCR5 did not affect HSV-1 replication, suggesting that other immune mechanisms are involved in the process of infection control | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clınica Medica. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clınica Medica.Belo Horizonte, MG, Brazil | en_US |
| Subject | CCL5 | en_US |
| Subject | Met-RANTES | en_US |
| Subject | Herpes virus | en_US |
| Subject | encephalitis | en_US |
| Subject | intravital microscopy | en_US |
| Embargo date | 3100-12-31 | |
