| Author | Krettli, Antoniana Ursine | |
| Author | Adebayo, Joseph Oluwatope | |
| Author | Krettli, Luisa Guimarães | |
| Access date | 2023-07-31T13:12:18Z | |
| Available date | 2023-07-31T13:12:18Z | |
| Document date | 2009 | |
| Citation | KRETTLI, Antoniana Ursine; ADEBAYO, Joseph Oluwatope; KRETTLI, Luisa Guimarães. Testing of natural products and synthetic molecules aiming at new antimalarials. Curr Drug Targets, v. 10, n. 3, p. 261-270, 2009. doi: 10.2174/138945009787581203. | en_US |
| ISSN | 1389-4501 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59848 | |
| Language | eng | en_US |
| Publisher | Bentham Science Publishers | en_US |
| Rights | restricted access | |
| Title | Testing of natural products and synthetic molecules aiming at new antimalarials | en_US |
| Type | Article | |
| DOI | 10.2174/138945009787581203 | |
| Abstract | The search for new antimalarials, which in the past relied on animal models, is now usually performed with cultures of Plasmodium falciparum (PF) blood parasites by evaluation of parasite growth inhibition. Field isolates of PF human malaria parasite, parasite strains and clones, well characterized for their susceptibility to chloroquine and other standard antimalarials are available for the in vitro tests. The simplest method to evaluate parasite growth is the determination of parasitemias in Giemsa stained blood smears through light microscopy. Other methodologies have proven to be more precise and allow mass screening of new compounds against PF blood stages, such as: (i) measuring the incorporation of radioactive hypoxanthine by the parasites; (ii) indirect colorimetric assays in which specific parasite enzyme activities, and histidine-rich protein II (HRP2) production are measured with the help of monoclonal antibodies; (iii) the haematin formation, and; (iv) assays using green fluorescent protein (GFP) in gene-expressing parasites. The advantages and disadvantages of the different in vitro screening methods, as well as the different in vivo models for antimalarial tests, are described in this review. Such tests can be used for the evaluation of medicinal plants, synthetic and hybrid molecules or drug combinations | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Imunopatologia. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Imunopatologia. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | University of Ilorin. Ilorin, Nigeria/ Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Malária Experimental e Humana. Belo Horizonte, MG, Brasil | en_US |
| Subject | Malaria | en_US |
| Subject | P. falciparum | en_US |
| Subject | antimalarials | en_US |
| Subject | experimental chemotherapy | en_US |
| Subject | in vitro tests | en_US |
| Subject | animal models | en_US |
| Subject | drug-resistance | en_US |
| Subject | hypoxanthine | en_US |
| Embargo date | 3100-12-31 | |
