| Author | Araújo, Márcio Sobreira Silva | |
| Author | Andrade, Renata Aline de | |
| Author | Avelar, Renato Sathler | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Andrade, Mariléia Chaves | |
| Author | Vianna, Leonardo Rocha | |
| Author | Mayrink, Wilson | |
| Access date | 2023-07-31T14:13:54Z | |
| Available date | 2023-07-31T14:13:54Z | |
| Document date | 2009 | |
| Citation | ARAÚJO, Márcio Sobreira Silva et al. T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune. Vaccine, v. 27, n. 7, p. 1008-1017. doi: 10.1016/j.vaccine.2008.11.104. | en_US |
| ISSN | 0264-410X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59849 | |
| Language | eng | en_US |
| Publisher | Elsevier Ltd. | en_US |
| Rights | restricted access | |
| Title | T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune (R) | en_US |
| Type | Article | |
| DOI | 10.1016/j.vaccine.2008.11.104 | |
| Abstract | It is generally accepted that distinct cytokine expression by the cellular immune response plays a critical role during the outcome of experimental as well as natural canine visceral Leishmaniasis (CVL). Despite the fact that immunoprophylaxis of CVL has become an important control strategy and protective immunity has been reported upon immunization with whole as well as purified Leishmania antigens, the cytokine profile of T-cells triggered by anti-CVL vaccines still remain to be determined. Herein, we have developed a cross-sectional analysis of German Shepherd dogs Submitted to vaccination protocols with Leishvaccine (n = 6) and Leishmune(R) (n = 6). Our data identified distinct immunological profiles elicited by Leishvaccine and Leishmune(R), with the Leishvaccine triggering a mixed, IFN-gamma and IL-4, cytokine pattern in addition to high levels of anti-Leishmania IgG 1, whereas the Leishmune(R) induced an immunological pattern characterized by enhanced levels of IFN-gamma, NO and anti-Leishmania chagasi IgG2. It was important to notice that despite the distinct immunological patterns triggered by Leishvaccine and Leishmune(R), the ability of both immumobiologicals to activate T-cell-derived IFN-gamma synthesis further suggesting their immunogenic potential against CVL. These findings added Support to Our hypothesis that both antigenic composition (whole antigen in Leishvaccine versus purified antigen in Leishmune(R)) as well as the adjuvant nature (BGC and saponin) used for the vaccine formulation May Count for the distinct activation pattern observed. (C) 2008 Elsevier Ltd. All rights reserved | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Estado de Minas Gerais. Polícia Militar. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | en_US |
| Subject | Canine visceral leishmaniasis | en_US |
| Subject | Leishvaccine | en_US |
| Subject | Leishmune (R) | en_US |
| Subject | Cytokines | en_US |
| Subject | Nitric oxide | en_US |
| Subject | IgG subclasses | en_US |
| Embargo date | 2099-12-31 | |
