Author | Junqueira, Caroline Furtado | |
Author | Polidoro, Rafael | |
Author | Castro, Guilherme | |
Author | Absalon, Sabrina | |
Author | Liang, Zhitao | |
Author | Santara, Sumit Sen | |
Author | Crespo, Ângela | |
Author | Pereira, Dhelio Batista | |
Author | Gazzinelli, Ricardo Tostes | |
Author | Dvorin, Jeffrey D. | |
Author | Lieberman, Judy | |
Access date | 2023-08-08T13:22:09Z | |
Available date | 2023-08-08T13:22:09Z | |
Document date | 2021 | |
Citation | JUNQUEIRA, Caroline Furtado et al. γδ T cells suppress Plasmodium falciparum blood-stage infection by direct killing and phagocytosis. Nature Immunology, v. 22, n. 3, p. 347-357, 2021. | en_US |
ISSN | 1529-2908 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59923 | |
Language | eng | en_US |
Publisher | Nature America Inc. | en_US |
Rights | restricted access | en_US |
Title | γδ T cells suppress Plasmodium falciparum blood-stage infection by direct killing and phagocytosis | en_US |
Type | Article | en_US |
DOI | 10.1038/s41590-020-00847-4 | |
Abstract | Activated Vγδ9Vδ2 (γδ2) T lymphocytes that sense parasite-produced phosphoantigens are expanded in Plasmodium falciparum-infected patients. Although previous studies suggested that γδ2 T cells help control erythrocytic malaria, whether γδ2 T cells recognize infected red blood cells (iRBCs) was uncertain. Here we show that iRBCs stained for the phosphoantigen sensor, butyrophilin 3A1 (BTN3A1). γδ2 T cells formed immune synapses and lysed iRBCs in a contact, phosphoantigen, BTN3A1 and degranulation-dependent manner, killing intracellular parasites. Granulysin released into the synapse lysed iRBCs and delivered death-inducing granzymes to the parasite. All intra-erythrocytic parasites were susceptible, but schizonts were most sensitive. A second protective γδ2 T cell mechanism was identified. In the presence of patient serum, γδ2 T cells phagocytosed and degraded opsonized iRBCs in a CD16-dependent manner, decreasing parasite multiplication. Thus, γδ2 T cells have two ways to control blood stage malaria – γδT cell antigen receptor (TCR)-mediated degranulation and phagocytosis of antibody-coated iRBCs. | en_US |
Affilliation | Program in Cellular and Molecular Medicine. Boston Children’s Hospital. USA / Department of Pediatrics. Harvard Medical School. USA / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Program in Cellular and Molecular Medicine. Boston Children’s Hospital. USA / Department of Pediatrics. Harvard Medical School. USA. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Department of Pediatrics. Harvard Medical School. USA / Division of Infectious Diseases. Boston Children’s Hospital. USA. | en_US |
Affilliation | Program in Cellular and Molecular Medicine. Boston Children’s Hospital. USA / Department of Pediatrics. Harvard Medical School. USA. | en_US |
Affilliation | Program in Cellular and Molecular Medicine. Boston Children’s Hospital. USA / Department of Pediatrics. Harvard Medical School. USA. | en_US |
Affilliation | Program in Cellular and Molecular Medicine. Boston Children’s Hospital. USA / Department of Pediatrics. Harvard Medical School. USA. | en_US |
Affilliation | Centro de Pesquisa em Medicina Tropical. Rondônia, RR, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil / Department of Medicine. University of Massachusetts Medical School. Worcester, MA,USA /F undação Oswaldo Cruz. Instituto René Rachou. Plataforma de Medicina Translacional. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Department of Pediatrics. Harvard Medical School. USA / Division of Infectious Diseases. Boston Children’s Hospital. USA. | en_US |
Affilliation | Program in Cellular and Molecular Medicine. Boston Children’s Hospital. USA / Department of Pediatrics. Harvard Medical School. USA. | en_US |
Subject | Malaria | en_US |
Subject | γδ T cell | en_US |
Subject | Cytotoxicity | en_US |
Subject | Granulysin | en_US |
Subject | Granzyme | en_US |
Subject | Butyrophilin | en_US |
Subject | Antibody-dependent phagocytosis | en_US |
Embargo date | 2099-12-31 | |