| Author | Diniz, Suelen Queiroz | |
| Author | Figueiredo, Maria Marta | |
| Author | Costa, Pedro Augusto Carvalho | |
| Author | Martins Filho, Olindo Assis | |
| Author | Carvalho, Andrea Teixeira | |
| Author | Pereira, Dhélio Batista | |
| Author | Tada, Mauro Shugiro | |
| Author | Afonso, Luis Carlos Crocco | |
| Author | Kohlhoff, Markus | |
| Author | Zani, Carlos Leomar | |
| Author | Oliveira, Fabiano | |
| Author | Antonelli, Lis Ribeiro | |
| Access date | 2023-08-16T19:23:16Z | |
| Available date | 2023-08-16T19:23:16Z | |
| Document date | 2023 | |
| Citation | DINIZ, Suelen Queiroz et al. Adenosine pathway regulates inflammation during Plasmodium vivax infection. Frontiers in Immunology, v. 14, p. 1193256, 2023. doi.org/10.3389/fimmu.2023.1193256. | en_US |
| ISSN | 1664-3224 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60122 | |
| Language | eng | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Title | Adenosine pathway regulates inflammation during Plasmodium vivax infection. | en_US |
| Type | Article | en_US |
| Abstract | Background: Plasmodium spp. infection triggers the production of inflammatory cytokines that are essential for parasite control, and conversely responsible for symptoms of malaria. Monocytes play a role in host defense against Plasmodium vivax infection and represent the main source of inflammatory cytokines and reactive oxygen species. The anti-inflammatory cytokine IL-10 is a key regulator preventing exacerbated inflammatory responses. Studies suggested that different clinical presentations of malaria are strongly associated with an imbalance in the production of inflammatory and anti-inflammatory cytokines.
Methods: A convenience sampling of peripheral blood mononuclear cells from Plasmodium vivax-infected patients and healthy donors were tested for the characterization of cytokine and adenosine production and the expression of ectonucleotidases and purinergic receptors.
Results: Here we show that despite a strong inflammatory response, monocytes also bear a modulatory role during malaria. High levels of IL-10 are produced during P. vivax infection and its production can be triggered in monocytes by P. vivax-infected reticulocytes. Monocytes express high levels of ectonucleotidases, indicating their important role in extracellular ATP modulation and consequently in adenosine production. Plasmatic levels of adenosine are not altered in patients experiencing acute malaria; however, their monocyte subsets displayed an increased expression of P1 purinergic receptors. In addition, adenosine decreases Tumor Necrosis Factor production by monocytes, which was partially abolished with the blockage of the A2a receptor.
Conclusion: Monocytes have a dual role, attempting to control both the P. vivax infection and the inflammatory response. Purinergic receptor modulators emerge as an untapped approach to ameliorate clinical malaria. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brazil. | en_US |
| Affilliation | Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Departamento de Ciências Biológicas. Instituto de Ciências Exatas e Biológicas. Ouro Preto, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Ricardo Tostes Gazzinelli | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases. National Institutes of Health. Rockville, MD, United States. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Plasmodium vivax | en_US |
| Subject | adenosine | en_US |
| Subject | ectonucleotidases | en_US |
| Subject | malaria | en_US |
| Subject | regulation | en_US |
