| Author | Cerávolo, Isabela Penna | |
| Author | Chaves, Andréa C. L. | |
| Author | Bonjardim, Cláudio A. | |
| Author | Ssibley, David | |
| Author | Romanha, Alvaro Jose | |
| Author | Gazzinelli, Ricardo Tostes | |
| Access date | 2023-09-12T19:14:26Z | |
| Available date | 2023-09-12T19:14:26Z | |
| Document date | 1999 | |
| Citation | CERÁVOLO, Isabela Penna et al. Replication of Toxoplasma gondii, but NotTrypanosoma cruzi, Is Regulated in Human Fibroblasts Activated with Gamma Interferon: Requirement of a Functional JAK/STAT Pathway. Infection and Immunity, v. 67, n. 5, p. 2233- 2240, 1999. | en_US |
| ISSN | 0019-9567 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60438 | |
| Language | eng | en_US |
| Publisher | ASM Journals | en_US |
| Rights | restricted access | |
| Title | Replication of Toxoplasma gondii, but NotTrypanosoma cruzi, Is Regulated in Human Fibroblasts Activated with Gamma Interferon: Requirement of a Functional JAK/STAT Pathway | en_US |
| Type | Article | |
| Abstract | To study the role of tryptophan degradation by indoleamine 2,3-dioxygenase (INDO) in the control of Trypanosoma cruzior Toxoplasma gondii replication, we used human fibroblasts and a fibrosarcoma cell line (2C4). The cells were cultured in the presence or absence of recombinant gamma interferon (rIFN-γ) and/or recombinant tumor necrosis factor alpha (rTNF-α) for 24 h and were then infected with either T. cruzi or T. gondii. Intracellular parasite replication was evaluated 24 or 48 h after infection. Treatment with rIFN-γ and/or rTNF-α had no inhibitory effect on T. cruzi replication. In contrast, 54, 73, or 30% inhibition of T. gondii replication was observed in the cells treated with rIFN-γ alone, rIFN-γ plus rTNF-α, or TNF-α alone, respectively. The replication of T. gondii tachyzoites in cytokine-activated cells was restored by the addition of extra tryptophan to the culture medium. Similarly,T. gondii tachyzoites transfected with bacterial tryptophan synthase were not sensitive to the microbiostatic effect of rIFN-γ. We also investigated the basis of the cytokine effect on parasite replication by using the three mutant cell lines B3, B9, and B10 derived from 2C4 and expressing defective STAT1α (signal transducer and activator of transcription), JAK2 (Janus family of cytoplasmic tyrosine kinases), or JAK1, respectively, three important elements of a signaling pathway triggered by rIFN-γ. We found that rTNF-α was able to induce low levels expression of INDO mRNA in the parental cell line, as well as the cell line lacking functional JAK2. In contrast to the parental cell line (2C4), rIFN-γ was not able to induce the expression of INDO mRNA or microbiostatic activity in any of the mutant cell lines. These findings indicate the essential requirement of the JAK/STAT pathway for the induction of high levels of INDO mRNA, tryptophan degradation, and the anti-Toxoplasma activity inside human nonprofessional phagocytic cells. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Viroses. Belo Horizonte, MG Brazil | en_US |
| Affilliation | Molecular Microbiology Department. Washington University. St. Louis, Missouri, USA | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquimica. Belo Horizonte, MG Brazil | en_US |
| Subject | Trypanosoma cruzi | en_US |
| Subject | Chagas disease | en_US |
| Embargo date | 2099-12-31 | |
