| Author | Chaves, Andrea Carla Leite | |
| Author | Cerávolo, Isabela Pena | |
| Author | Estanislau, Juliana de Assis Silva Gomes | |
| Author | Zani, Carlos Leomar | |
| Author | Romanha, Alvaro José | |
| Author | Gazzinelli, Ricardo Tostes | |
| Access date | 2023-09-20T17:14:25Z | |
| Available date | 2023-09-20T17:14:25Z | |
| Document date | 2001 | |
| Citation | CHAVES, Andrea Carla Leite et al. IL-4 and IL-13 regulate the induction of indoleamine 2,3-dioxygenase activity and the control of Toxoplasma gondii replication in human fibroblasts activated with IFN-gamma. Eur J Immunol., v. 31, n. 2, p. 333-344, 2001. doi: 10.1002/1521-4141(200102)31:2<333::aid-immu333>3.0.co;2-x. | en_US |
| ISSN | 0014-2980 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60509 | |
| Language | eng | en_US |
| Publisher | Wiley-VCH | en_US |
| Rights | restricted access | |
| Title | IL-4 and IL-13 regulate the induction of indoleamine 2,3-dioxygenase activity and the control of Toxoplasma gondii replication in human fibroblasts activated with IFN-gamma. | en_US |
| Type | Article | |
| Abstract | The ability of up-regulatory [recombinant (r) IFN- , rIFN- and rTNF- ¼] and down-regulatory (rIL-4, rIL-10 and rIL-13) cytokines to control the expression of indoleamine 2,3-dioxygenase (INDO) and anti-Toxoplasma activity in the human fibrosarcoma cell line 2C4 was evaluated. Activation of fibroblasts with rIFN- , rIFN- and rTNF- ¼resulted in augmentation of INDO expression and activity leading to 40.0, 25.0 and 27.0 % inhibition of tachyzoite growth, respectively. An additive effect was observed when host cells were incubated with rIFN- plus rTNF- ¼. With regard to the down-regulatory cytokines we observed that IL-4 as well as IL-13, but not IL-10, induced significant inhibition of IFN- -induced control of parasite replication, INDO mRNA expression and tryptophan catabolism. Similarly, IL-4 but not IL-10 inhibited the cell surface expression of HLA-DR and CD2 induced by IFN- . Consistent with these findings we were able to detect by reverse transcription-PCR the expression of mRNA for different chains of IL-4 and IL-13 receptors (IL-4R ¼, IL-13R ¼1 and IL-13R ¼2) but not for IL-10 receptor in the 2C4 and other human lung fibroblast cell lines (LL24 and MRC5). Together our results indicate that IL-4 and IL-13, but not IL-10, are implicated in the negative regulation of IFN- -induced anti-Toxoplasma activity in human cells from fibroblast lineage. | en_US |
| Affilliation | Department of Biochemistry and Immunology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Biochemistry and Immunology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Biochemistry and Immunology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Subject | Human fibroblast | en_US |
| Subject | Toxoplasma gondii | en_US |
| Subject | Indoleamine 2,3-dioxygenase | en_US |
| Subject | Microbiostasis | en_US |
| Subject | Cytokine | en_US |
| Embargo date | 2099-12-31 | |
