Author | Fernandes, Cloé Duarte | |
Author | Murta, Silvane Maria Fonseca | |
Author | Cerávolo, Isabela Penna | |
Author | Krug, Luciano Percival | |
Author | Vidigal, Pedro G | |
Author | Steindel, Mario | |
Author | Nardi, Nanci | |
Author | Romanha, Alvaro José | |
Access date | 2023-09-20T17:24:00Z | |
Available date | 2023-09-20T17:24:00Z | |
Document date | 1997 | |
Citation | FERNANDES, Cloé Duarte et al. Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil. Mem. Inst. Oswaldo Cruz, v. 92, n. 3, p. 343-351, 1997. doi.org/10.1590/S0074-02761997000300008 | en_US |
ISSN | 0074-0276 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/60510 | |
Language | eng | en_US |
Publisher | Ministério da Saúde | en_US |
Rights | open access | en_US |
Title | Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil | en_US |
Type | Article | en_US |
Abstract | Thirty-five Trypanosoma cruzi strains were isolated from chronic chagasic patients, triatomines and opossums from different municipalities of the State of Rio Grande do Sul. Parasites were characterized by means of mice infectivity, enzyme electrophoresis and randomly amplified polymorphic DNA (RAPD) analysis. Twenty-nine strains were isolated from chagasic patients, 4 from triatomines (2 from Triatoma infestans and 2 from Panstrongylus megistus) and 2 from opossums Didelphis albiventris. Thirty-three T. cruzi strains were of low and 2 strains of high virulence in mice. Both virulent strains were isolated from P. megistus. Isoenzyme analysis of the strains showed 3 different zymodemes. Eleven strains isolated from chagasic patients and 2 from D. albiventris were Z2. Eighteen strains from patients and 2 from T. infestans were ZB and 2 T. cruzi strains isolated from P. megistus were Z1. RAPD profiles obtained with 4 random primers showed a high genetic heterogeneity of the T. cruzi strains. Zymodeme 2 and ZB strains were the more polymorphic. A band sharing analysis of the RAPD profiles of Z2 and ZB strains using 3 primers, showed a very low percentage of shared bands, 20% among 13 ZB strains and 14% among 13 Z2 strains. According to the isoenzyme results, 3 T. cruzi populations were present in State of Rio Grande do Sul. Zymodeme 2 and ZB strains were found infecting man (domiciliar transmission cycle) whereas Z1 strains were found infecting the sylvatic vector P. megistus. | en_US |
Affilliation | SSMA/RS. Instituto de Pesquisas Biológicas. Porto Alegre, RS, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brasil | en_US |
Affilliation | Universidade Federal do Rio Grande do Sul. Departamento de Genética. Porto Alegre, RS, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil | en_US |
Subject | Chagas’ disease | en_US |
Subject | Trypanosoma cruzi | en_US |
Subject | randomly amplified polymorphic DNA | en_US |
Subject | isoenzyme characterization | en_US |
Subject | epidemiology | en_US |