Author | Almeida, Daniela Santos | |
Author | Paz, Lucas Nogueira | |
Author | Oliveira, Daiana Santos de | |
Author | Silva, Danielle Nascimento | |
Author | Ristow, Paula | |
Author | Hamond, Camila | |
Author | Costa, Federico | |
Author | Portela, Ricardo Wagner | |
Author | Lima, Alessandra Estrela | |
Author | Pinna, Melissa Hanzen | |
Access date | 2023-10-05T15:59:10Z | |
Available date | 2023-10-05T15:59:10Z | |
Document date | 2019 | |
Citation | ALMEIDA, Daniela Santos et al. investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. PLoS ONE, v. 14, n. 5, p. 1-13, 2019. | en_US |
ISSN | 1932-6203 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/60719 | |
Sponsorship | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). | en_US |
Language | eng | en_US |
Publisher | Public Library of Science | en_US |
Rights | open access | en_US |
Subject in Portuguese | Leptospira | en_US |
Subject in Portuguese | Infecção crônica | en_US |
Subject in Portuguese | Ovelha | en_US |
Title | Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse | en_US |
Type | Article | en_US |
DOI | 10.1371/journal.pone.0217391 | |
Abstract | The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse.In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with
leptospirosis. | en_US |
Affilliation | Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil. | en_US |
Affilliation | Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Federal da Bahia. Instituto de Biologia. Laboratório de Bacteriologia e Saúde. Salvador, BA, Brasil. | en_US |
Affilliation | Plataforma de Salud Animal. Instituto Nacional de Investigacio´n Agropecuaria, INIA La Estanzuela. Colonia, Uruguay. | en_US |
Affilliation | Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil. | en_US |
Subject | Leptospira | en_US |
Subject | Chronic infection | en_US |
Subject | Sheep | en_US |
DeCS | Leptospira | en_US |
DeCS | Infecção Persistente | en_US |
DeCS | Ovinos | en_US |