| Author | Kramer, Susanne | |
| Author | Karolak, Natalia Katarzyna | |
| Author | Odenwald, Johanna | |
| Author | Gabiatti, Bernardo | |
| Author | Londoño, Paula Andrea Castañeda | |
| Author | Zavrelová, Anna | |
| Author | Freire, Eden Ribeiro | |
| Author | Almeida, Kayo Schemiko | |
| Author | Braune, Silke | |
| Author | Moreira, Claudia | |
| Author | Eder, Amelie | |
| Author | Goos, Carina | |
| Author | Field, Mark | |
| Author | Carrington, Mark | |
| Author | Holetz, Fabiola | |
| Author | Górna, Maria Wiktoria | |
| Author | Zoltner, Martin | |
| Access date | 2023-10-10T13:30:51Z | |
| Available date | 2023-10-10T13:30:51Z | |
| Document date | 2023 | |
| Citation | KRAMER, Susanne et al. A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research. v. 51, n. 14, p. 7520-7540, 2023. | en_US |
| ISSN | 1362-4962 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60752 | |
| Language | por | en_US |
| Publisher | Oxford University Press | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | mRNA | en_US |
| Title | A unique mRNA decapping complex in trypanosomes | en_US |
| Type | Article | en_US |
| Abstract | Removal of the mRNA 5' cap primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme Dcp2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5' -3'exoribonuclease Xrn1. Kinetoplastida lack Dcp2 orthologues but instead rely on the ApaH-like phosphatase ALPH1 for decapping. ALPH1 is composed of a catalytic domain flanked by C- and N-terminal extensions. We show that T. brucei ALPH1 is dimeric in vitro and functions within a complex composed of the trypanosome Xrn1 ortholog XRNA and four proteins unique to Kinetoplastida, including two RNA- binding proteins and a CMGC-family protein kinase. All ALPH1-associated proteins share a unique and dynamic localization to a structure at the posterior pole of the cell, anterior to the microtubule plus ends. XRNA affinity capture in T. cruzi recapitulates this interaction network. The ALPH1 N-terminus is not required for viability in culture, but essential for posterior pole localization. The C-terminus, in contrast, is required for localization to all RNA granule types, as well as for dimerization and interactions with XRNA and the CMGC kinase, suggesting possible regulatory mechanisms. Most significantly, the trypanosome decapping complex has a unique composition, differentiating the process from opisthokonts. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. | en_US |
| Affilliation | Biological and Chemical Research Centre. Department of Chemistry. University of Warsaw. Warsaw, Poland. / Nencki Institute of Experimental Biology. Polish Academy of Sciences. Warsaw, Poland. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. | en_US |
| Affilliation | Department of Parasitology. Faculty of Science. Charles University in Prague. Biocev, Vestec, Czech Republic. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. | en_US |
| Affilliation | Biocenter. University of Würzburg. Würzburg, Germany. | en_US |
| Affilliation | School of Life Sciences. University of Dundee. Dundee, UK. / Institute of Parasitology. Biology Center. Czech Academy of Sciences. České Budějovice, Czech Republic. | en_US |
| Affilliation | Department of Biochemistry. University of Cambridge. Cambridge, United Kingdom. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Biological and Chemical Research Centre. Department of Chemistry. University of Warsaw. Warsaw, Poland. | en_US |
| Affilliation | Department of Parasitology. Faculty of Science. Charles University in Prague. Biocev, Vestec, Czech Republic. | en_US |
| Subject | Trypanosome | en_US |
| Subject | RNA, Messenger | en_US |
| Subject in Spanish | ARN Mensajero | en_US |
| Subject in French | ARN messager | en_US |
| DeCS | Trypanosoma | en_US |
| DeCS | RNA Mensageiro | en_US |
| DeCS | Agentes de Decapagem | en_US |
