| Author | Rizzo, Juliana | |
| Author | Trottier, Adèle | |
| Author | Moyrand, Frédérique | |
| Author | Coppée, Jean-Yves | |
| Author | Maufrais, Corinne | |
| Author | Zimbres, Ana Claudia G. | |
| Author | Dang, Thi Tuong Vi | |
| Author | Alanio, Alexandre | |
| Author | Desnos-Ollivier, Marie | |
| Author | Mouyna, Isabelle | |
| Author | Péhau-Arnaude, Gérard | |
| Author | Commere, Pierre-Henri | |
| Author | Novault, Sophie | |
| Author | Ene, Iuliana V. | |
| Author | Nimrichter, Leonardo | |
| Author | Rodrigues, Marcio Lourenço | |
| Author | Janbon, Guilhem | |
| Access date | 2023-10-16T16:18:48Z | |
| Available date | 2023-10-16T16:18:48Z | |
| Document date | 2023 | |
| Citation | RIZZO, Juliana et al. Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans. mBio. v. 14, n. 4, p. 1-22, 2023. | en_US |
| ISSN | 2150-7511 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60811 | |
| Language | por | en_US |
| Publisher | Public Library of Science | en_US |
| Rights | open access | en_US |
| Title | Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans | en_US |
| Type | Article | en_US |
| Abstract | Resistance to fluconazole (FLC), the most widely used antifungal drug, is typically achieved by altering the azole drug target and/or drug efflux pumps. Recent reports have suggested a link between vesicular trafficking and antifungal resistance. Here, we identified novel Cryptococcus neoformans regulators of extracellular vesicle (EV) biogenesis that impact FLC resistance. In particular, the transcription factor Hap2 does not affect the expression of the drug target or efflux pumps, yet it impacts the cellular sterol profile. Subinhibitory FLC concentrations also downregulate EV production. Moreover, in vitro spontaneous FLC-resistant colonies showed altered EV production, and the acquisition of FLC resistance was associated with decreased EV production in clinical isolates. Finally, the reversion of FLC resistance was associated with increased EV production. These data suggest a model in which fungal cells can regulate EV production in place of regulating the drug target gene expression as a first line of defense against antifungal assault in this fungal pathogen. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. / Institut Pasteur. Paris, France. / Université Paris Cité. USR 3756 IP CNRS, HUB Bioinformatique et Biostatistique. Paris, France. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Centre National de Référence Mycoses Invasives et Antifongiques. Groupe de recherche Mycologie Translationnelle. Département de Mycologie. Paris, France. / Laboratoire de parasitologie-mycologie, AP-HP. Hôpital Saint-Louis. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Centre National de Référence Mycoses Invasives et Antifongiques. Groupe de recherche Mycologie Translationnelle. Département de Mycologie. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Plateforme de Bio-Imagerie Ultrastructurale. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Cytometry and Biomarkers. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Cytometry and Biomarkers. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Fungal Heterogeneity Group. Paris, France. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. | en_US |
| Subject | Cryptococcus neoformans | en_US |
| Subject | Extracellular Vesicles | en_US |
| Subject | Drug Resistance, Microbial | en_US |
| Subject | Fluconazole | en_US |
| Subject | Transcription Factors | en_US |
| Subject in Spanish | Cryptococcus neoformans | en_US |
| Subject in Spanish | Vesículas Extracelulares | en_US |
| Subject in Spanish | Farmacorresistencia Microbiana | en_US |
| Subject in Spanish | Fluconazol | en_US |
| Subject in Spanish | Factores de Transcripción | en_US |
| Subject in French | Cryptococcus neoformans | en_US |
| Subject in French | Vésicules extracellulaires | en_US |
| Subject in French | Résistance microbienne aux médicaments | en_US |
| Subject in French | Fluconazole | en_US |
| Subject in French | Facteurs de transcription | en_US |
| DeCS | Cryptococcus neoformans | en_US |
| DeCS | Vesículas Extracelulares | en_US |
| DeCS | Resistência Microbiana a Medicamentos | en_US |
| DeCS | Fluconazol | en_US |
| DeCS | Fatores de Transcrição | en_US |
