| Author | Nascimento, Marcelo Reis do | |
| Author | Souza, Rodrigo O. Silva de | |
| Author | Silva Junior, Alexander L. | |
| Author | Lima, Emerson Silva | |
| Author | Gonçalves, Marilda Souza | |
| Author | Moura Neto, José Pereira de | |
| Access date | 2023-10-19T18:26:43Z | |
| Available date | 2023-10-19T18:26:43Z | |
| Document date | 2021 | |
| Citation | NASCIMENTO, Marcelo Reis do et al. GSTP1 rs1695 and rs1871042, and SOD2 rs4880 as molecular markers of lipid peroxidation in blood storage. Blood Transfus, v. 19, n. 4, p. 1-8, 2021. | en_US |
| ISSN | 2385-2070 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60857 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). | en_US |
| Language | eng | en_US |
| Publisher | SIMTIPRO Srl | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | GSTP1 | en_US |
| Subject in Portuguese | SOD2 | en_US |
| Subject in Portuguese | Peroxidação lipídica | en_US |
| Subject in Portuguese | Armazenamento de sangue | en_US |
| Subject in Portuguese | Lesões de armazenamento | en_US |
| Title | GSTP1 rs1695 and rs1871042, and SOD2 rs4880 as molecular markers of lipid peroxidation in blood storage | en_US |
| Type | Article | en_US |
| DOI | 10.2450/2020.0062-20 | |
| Abstract | Background: Red blood cells (RBC) are subject to oxidative stress by reactive oxygen species (ROS) during storage. Molecular characterisation of oxidative stress in stored RBC, which may also occur in other blood components during long periods of storage, is rare. Materials and methods: Our study included 45 healthy RBC donors recruited in Brazil. Blood was collected into standard Grifols® Triple Bags containing CPD SAG-M. Haematological values, biochemical data, and oxidative stress markers were assessed weekly during storage until 42 days after collection. GSTM1 and GSTT1 were determined by multiplex-polymerase chain reaction (PCR), while GSTP1 rs1695 and rs1871042, CAT rs1001179, and SOD2 rs4880 were evaluated by real-time PCR. Results: A direct proportional relationship was found between storage time and levels of ROS and thiobarbituric acid reactive substances (TBARS, indicators of lipid peroxidation) (p<0.001). These parameters were indirectly proportional to ABTS values (p<0.001). The plasma concentration of TBARS was associated with GSTP1 303AG/GG, GSTP1 -16CT/TT, and SOD2 47CT/TT genotypes. Single-nucleotide polymorphisms at the CAT C-262T gene were not associated with TBARS, nor were oxidative markers of ROS. Discussion: Prolonged storage may result in the onset of erythrocyte deterioration. Our results clearly indicate that erythrocytes are capable of attenuating ROS for 2 weeks of storage. We observed an association between elevated TBARS levels and the presence of GSTP1 and SOD2 variants in stored RBC. Although notable for heterozygous variants, this association was even stronger for the homozygous variants GSTP1 rs1695 (303GG), GSTP1 rs1871042 (-16TT), and SOD2 rs4880 (47TT). These findings accentuate the importance of genetic factors in storage lesions and will expand our understanding and consideration of endogenous and exogenous causes in improving clinical treatment with blood transfusions. | en_US |
| Affilliation | Fundação Hospitalar do Amazonas. Hematologia e Hemoterapia. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil. | en_US |
| Affilliation | Fundação Hospitalar do Amazonas. Hematologia e Hemoterapia. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Hospitalar do Amazonas. Hematologia e Hemoterapia. Manaus, AM, Brasil. | en_US |
| Subject | GSTP1 | en_US |
| Subject | SOD2 | en_US |
| Subject | Lipid peroxidation | en_US |
| Subject | Blood storage | en_US |
| Subject | Storage lesions | en_US |
| DeCS | Glutationa S-Transferase pi | en_US |
| DeCS | Armazenamento de Sangue | en_US |
