| Author | Portis, Igor Godinho | |
| Author | Lima, Patrícia de Sousa | |
| Author | Paes, Rodrigo Almeida | |
| Author | Oliveira, Lucas Nojosa | |
| Author | Pereira, Christie Ataídes | |
| Author | Parente-Rocha, Juliana Alves | |
| Author | Pereira, Maristela | |
| Author | Nosanchuk, Joshua Daniel | |
| Author | Soares, Célia Maria de Almeida | |
| Access date | 2023-10-29T15:49:19Z | |
| Available date | 2023-10-29T15:49:19Z | |
| Document date | 2020 | |
| Citation | PORTIS, Igor Godinho et al. Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin. Microbiological Research, v. 239, p. 1-9, Oct. 2020. | en_US |
| ISSN | 0944-5013 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60981 | |
| Sponsorship | This work was supported by grants from Instituto Nacional de Ciência e Tecnologia de Estratégias de Interação Patógeno-Hopedeiro - Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG). CMAS, MP and JAPR are fellow researchers from Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq. JDN is partially supported by NIHAI52733. RAP is supported by CNPq grants (449184/2014-5 and 305487/2015-9). IGP, RAP, LNO and CAP and were supported by fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES. The authors wish to thank Laurine Lacerda Pigosso for the technical assistance in the initial development of the Project. | en_US |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | open access | en_US |
| Title | Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin | en_US |
| Type | Article | en_US |
| DOI | 10.1016/j.micres.2020.126524 | |
| Abstract | Paracoccidioidomycosis is a highly prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. Copper is essential for eukaryotes and bacteria. This micronutrient is used in many vital biochemical processes, although metal excess levels can be toxic for organisms. Pathways underlying copper overload are poorly understood in members of the Paracoccidioides complex. The responses of Paracoccidioides lutzii yeast cells to copper overload were here evaluated. The results showed that under copper overload, cells presented a dark brown pigment, identified as melanin. Proteomic analyses identified mainly the accumulation of proteins related to amino acids metabolism, ergosterol synthesis and melanin production, suggesting that P. lutzii responds to copper overload by changing aspects of its metabolism and also plasma membrane and cell wall remodeling. Proteomic data were confirmed by biochemical analysis. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | en_US |
| Affilliation | Albert Einstein College of Medicine. Department of Medicine. New York City, NY, USA. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | en_US |
| Subject | Cellular defense | en_US |
| Subject | Copper overload | en_US |
| Subject | Fungi | en_US |
| Subject | Proteome | en_US |
| e-ISSN | 1618-0623 | |
