| Author | Silva, Anielly Sarana da | |
| Author | Campos, Gabriel Montenegro de | |
| Author | Villanova, Marcia Guimarães | |
| Author | Bezerra, Rafael Dos Santos | |
| Author | Santiago, Luciana Maria Mendes | |
| Author | Haddad, Rodrigo | |
| Author | Covas, Dimas Tadeu | |
| Author | Giovanetti, Marta | |
| Author | Alcantara, Luiz Carlos Junior | |
| Author | Elias, Maria Carolina | |
| Author | Sampaio, Sandra Coccuzzo | |
| Author | Kashima, Simone | |
| Author | Slavov, Svetoslav Nanev | |
| Access date | 2023-11-07T18:02:02Z | |
| Available date | 2023-11-07T18:02:02Z | |
| Document date | 2023 | |
| Citation | SILVA, Anielly Sarana da et al. Human Pegivirus-1 Detection and Genotyping in Brazilian Patients with Fulminant Hepatitis. Pathogens, v. 12, n. 9, p. 1-8, 2023. | en_US |
| ISSN | 2076-0817 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/61074 | |
| Language | eng | en_US |
| Publisher | MDPI AG | en_US |
| Rights | restricted access | |
| Title | Human Pegivirus-1 Detection and Genotyping in Brazilian Patients with Fulminant Hepatitis | en_US |
| Type | Article | |
| DOI | 10.3390/pathogens12091122 | |
| Abstract | Fulminant hepatitis is a severe clinical disease characterized by a marked decline in liver function and encephalopathy. In a previous survey, using metagenomics in a group of 27 patients with this clinical condition, we observed an expressive quantity of reads of the Human pegivirus-1 (HPgV-1). Therefore, the objective of this study was to evaluate the frequency, molecular features, and HPgV-1 circulating genotypes in patients with fulminant hepatitis. After testing the collected plasma samples, we discovered twelve samples (44.4%) that were positive for HPgV-1 RNA (using both real-time and nested PCR). The positive samples presented a mean cycle threshold (Ct) of 28.5 (±7.3). Genotyping assignments revealed that all HPgV-1 positive samples belonged to the HPgV-1 genotype 2 (both subgenotypes 2A and 2B were identified). Although HPgV-1 is considered a commensal virus, little is known regarding its prevalence and genotypes in cases of fulminant hepatitis. More research is needed to understand whether HPgV-1 can be implicated in clinical disorders and infectious diseases. | en_US |
| Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Department of Gastroenterology. University Hospital. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Faculty of Ceilândia. University of Brasília. Brasília, DF, Brazil. | en_US |
| Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Instituto Rene Rachou. Fundação Oswaldo Cruz. Belo Horizonte, MG, Brazil / Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy. | en_US |
| Affilliation | Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy. | en_US |
| Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
| Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
| Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil / Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
| Subject | HPgV-1 | en_US |
| Subject | Fulminant hepatitis | en_US |
| Subject | Genotyping | en_US |
| Subject | Human pegvirus-1 | en_US |
| Subject | qPCR | en_US |
| Embargo date | 2030-12-31 | |
