Author | Dallastella, Marianna | |
Author | Oliveira, Willian Klassen de | |
Author | Rodrigues, Marcio Lourenço | |
Author | Goldenberg, Samuel | |
Author | Alves, Lysangela Ronalte | |
Access date | 2023-11-07T18:08:10Z | |
Available date | 2023-11-07T18:08:10Z | |
Document date | 2023 | |
Citation | DALLASTELLA, Marianna et al. The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles. Front. Cell. Infect. Microbiol. V.13, n. 1247329., p. 1-12, 2023. | en_US |
ISSN | 2235-2988 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/61075 | |
Language | por | en_US |
Publisher | Frontiers Media | en_US |
Rights | open access | en_US |
Title | The characterization of RNAbinding proteins and RNA metabolism-related proteins in fungal extracellular vesicles | en_US |
Type | Article | en_US |
DOI | 10.3389/fcimb.2023.1247329 | |
Abstract | RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi contain a variety of proteins involved in RNA metabolism. These include RNA helicases, which play essential roles in RNA synthesis, folding, and degradation. Aminoacyl-tRNA synthetases, responsible for acetylating tRNA molecules, are also enriched in EVs, suggesting a possible link between these enzymes and tRNA fragments detected in EVs. Proteins with canonical RNA-binding domains interact with proteins and RNA, such as the RNA Recognition Motif (RRM), Zinc finger, and
hnRNP K-homology (KH) domains. Polyadenylate-binding protein (PABP) plays a critical role in the regulation of gene expression by binding the poly(A) tail of messenger RNA (mRNA) and facilitating its translation, stability, and localization, making it a key factor in post-transcriptional control of gene expression. The presence of proteins related to the RNA life cycle in EVs from different fungal species suggests a conserved mechanism of EV cargo packing. Various models have been proposed for selecting RNA molecules for release into EVs. Still, the actual loading processes are unknown, and further molecular characterization of these proteins may provide insight into the mechanism of RNA sorting into EVs. This work reviews the current knowledge of RBPs and proteins related to RNA metabolism in EVs derived from distinct fungi species, and presents an analysis of proteomic datasets through GO term and orthology analysis, Our investigation identified orthologous proteins in fungal EVs on different fungal species. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. / Research Center in Infectious Diseases. Division of Infectious Disease and Immunity CHU de Quebec Research Center. University Laval, Québec City, Canada. | en_US |
Subject | Extracellular Vesicles | en_US |
Subject | RNA-Binding Proteins | en_US |
Subject | Metabolism | en_US |
Subject | Proteomics | en_US |
Subject | Cell Communication | en_US |
Subject in Spanish | Vesículas Extracelulares | en_US |
Subject in Spanish | Proteínas de Unión al ARN | en_US |
Subject in Spanish | Metabolismo | en_US |
Subject in Spanish | Proteómica | en_US |
Subject in Spanish | Comunicación Celular | en_US |
Subject in French | Vésicules extracellulaires | en_US |
Subject in French | Protéines de liaison à l'ARN | en_US |
Subject in French | Métabolisme | en_US |
Subject in French | Protéomique | en_US |
Subject in French | Communication cellulaire | en_US |
DeCS | Vesículas Extracelulares | en_US |
DeCS | Proteínas de Ligação a RNA | en_US |
DeCS | Metabolismo | en_US |
DeCS | Proteômica | en_US |
DeCS | Comunicação Celular | en_US |