| Author | Silveira, Brysa M. | |
| Author | Ribeiro, Tiago O. | |
| Author | Freitas, Railane S. | |
| Author | Carreira, Ana C. O. | |
| Author | Gonçalves, Marilda Souza | |
| Author | Sogayar, Mari | |
| Author | Meye, Roberto | |
| Author | Birbrair, Alexander | |
| Author | Fortuna, Vitor | |
| Access date | 2023-11-23T13:47:51Z | |
| Available date | 2023-11-23T13:47:51Z | |
| Document date | 2022 | |
| Citation | SILVEIRA, Brysa M. et al. Secretome from human adipose-derived mesenchymal stem cells promotes blood vessel formation and pericyte overage in experimental skin repair. Plos One, v. 17, n. 12, p. 1-21, 2022. | en_US |
| ISSN | 1932-6203 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/61423 | |
| Language | eng | en_US |
| Publisher | Public Library of Science | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Secretoma | en_US |
| Subject in Portuguese | Tecido adiposo | en_US |
| Subject in Portuguese | Células-tronco mesenquimais | en_US |
| Subject in Portuguese | Vaso sanguíneo | en_US |
| Subject in Portuguese | Pericito | en_US |
| Subject in Portuguese | Reparação de pele | en_US |
| Title | Secretome from human adipose-derived mesenchymal stem cells promotes blood vessel formation and pericyte overage in experimental skin repair | en_US |
| Type | Article | en_US |
| DOI | 10.1371/journal.pone.0277863 | |
| Abstract | Human adipose tissue-derived stem cells (hASC) secretome display various therapeutically relevant effects in regenerative medicine, such as induction of angiogenesis and tissue repair. The benefits of hASC secretome are primarily orchestrated by trophic factors that mediate autocrine and paracrine effects in host cells. However, the composition and the innate characteristics of hASC secretome can be highly variable depending on the culture conditions. Here, we evaluated the combined effect of serum-free media and hypoxia preconditioning on the hASCs secretome composition and biological effects on angiogenesis and wound healing. The hASCs were cultured in serum-free media under normoxic (NCM) or hypoxic (HCM) preconditioning. The proteomic profile showed that pro- and anti-antiangiogenic factors were detected in NCM and HCM secretomes. In vitro studies demonstrated that hASCs secretomes enhanced endothelial proliferation, survival, migration, in vitro tube formation, and in vivo Matrigel plug angiogenesis. In a full-thickness skin-wound mouse model, injection of either NCM or HCM significantly accelerated the wound healing. Finally, hASC secretomes were potent in increasing endothelial density and vascular coverage of resident pericytes expressing NG2 and nestin to the lesion site, potentially contributing to blood vessel maturation. Overall, our data suggest that serum-free media or hypoxic preconditioning enhances the vascular regenerative effects of hASC secretome in a preclinical wound healing model. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia. São Paulo, SP, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Escola de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Patologia. Belo Horizonte, MG, Brasil / Department of Radiology. Columbia University Medical Center. New York, NY, United States of America / Department of Dermatology. School of Medicine and Public Health. University of Wisconsin-Madison. Madison, Wisconsin, United States of America. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas. Salvador, BA, Brasil. | en_US |
| Subject | Secretome | en_US |
| Subject | Tecido adiposo | en_US |
| Subject | Células-tronco mesenquimais | en_US |
| Subject | Blood vessel | en_US |
| Subject | Pericyte | en_US |
| Subject | Skin repair | en_US |
| DeCS | Secretoma | en_US |
| DeCS | Tecido Adiposo | en_US |
| DeCS | Células-Tronco Mesenquimais | en_US |
