| Author | Rabahi, Marcelo Fouad | |
| Author | Conceição, Emilyn Costa | |
| Author | Paiva, Luísa Oliveira de | |
| Author | Souto, Marcos Vinícius Muniz Lemes | |
| Author | Sisco, Maria Carolina | |
| Author | Waard, Jacobus de | |
| Author | Caldas, Paulo Cesar de Souza | |
| Author | Fandinho, Fatima | |
| Author | Ramos, Jesus Pais | |
| Author | Carvalho, Luciana Distasio de | |
| Author | Campos, Carlos Eduardo Dias | |
| Author | Lima, Karla Valéria Batista | |
| Author | Silva, Sandro Patroca da | |
| Author | Sharma, Abhinav | |
| Author | Robledo, Jaime | |
| Author | Paezi, Uriel Alonso Hurtado | |
| Author | Duarte, Rafael Silva | |
| Author | Silva, Marlei Gomes da | |
| Author | Gomes, Lia Lima | |
| Author | Vasconcellos, Sidra Ezidio Gonçalves | |
| Author | Uwezeye, Cecile | |
| Author | Jong, Bouke C. de | |
| Author | Junqueira-Kipnis, Ana Paula | |
| Author | Suffys, Philip Noel | |
| Access date | 2023-11-27T00:39:23Z | |
| Available date | 2023-11-27T00:39:23Z | |
| Document date | 2020 | |
| Citation | RABAHI, Marcelo Fouad et al. Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, n. 85, p. 1-7, Nov. 2020. | en_US |
| ISSN | 1567-1348 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/61471 | |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | open access | |
| Title | Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil | en_US |
| Type | Article | |
| DOI | 10.1016/j.meegid.2020.104550 | |
| Abstract | Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case. | en_US |
| Affilliation | Universidade Federal de Goiás. Hospital das Clínicas. Setor Leste Universitário. Goiânia, GO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Programa de Pós-graduação em Pesquisa Clínica e Doenças Infecciosas. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal de Goiás. Hospital das Clínicas. Setor Leste Universitário. Goiânia, GO, Brasil. | en_US |
| Affilliation | Universidade Federal de Goiás. Hospital das Clínicas. Setor Leste Universitário. Goiânia, GO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidad de Las Américas. Facultad de Ciencias de La Salud. One Health Research Group. Quito, Ecuador. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sérgio Arouca. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sérgio Arouca. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sérgio Arouca. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sérgio Arouca. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sérgio Arouca. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brasil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brasil. | en_US |
| Affilliation | International Institute of InformationTechnology. Bangalore (IIIT-B). Department of Data Science. Bangalore, India. | en_US |
| Affilliation | Corporación para Investigaciones Biológicas. Medellín, Colombia. | en_US |
| Affilliation | Corporación para Investigaciones Biológicas. Medellín, Colombia. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium. | en_US |
| Affilliation | Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium. | en_US |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Mycobacterium tuberculosis var. africanum | en_US |
| Subject | Tuberculosis | en_US |
| Subject | Molecular epidemiology | en_US |
| Subject | Genome | en_US |
| Subject | Case report | en_US |
| Subject | Brazil | en_US |
| e-ISSN | 1567-7257 | |
