| Author | Castro, Isabela Silva de | |
| Author | Granato, Alessandra | |
| Author | Mariante, Rafael Meyer | |
| Author | Lima, Marco Antonio | |
| Author | Leite, Ana Claudia Celestino | |
| Author | Espindola, Otávio de Melo | |
| Author | Pise-Masison, Cynthia A. | |
| Author | Franchini, Genoveffa | |
| Author | Linden, Rafael | |
| Author | Echevarria-Lima, Juliana | |
| Access date | 2023-11-30T23:37:34Z | |
| Available date | 2023-11-30T23:37:34Z | |
| Document date | 2023 | |
| Citation | CASTRO, Isabela Silva de et al. HTLV-1 p12 modulates the levels of prion protein (PrPC) in CD4+ T cells. Frontiers in microbiology, v. 14, p. 1-13, Aug. 2023. | en_US |
| ISSN | 1664-302X | |
| URI | https://www.arca.fiocruz.br/handle/icict/61581 | |
| Language | eng | en_US |
| Publisher | Frontiers Research Foundation | en_US |
| Rights | open access | |
| Title | HTLV-1 p12 modulates the levels of prion protein (PrPC) in CD4+ T cells | en_US |
| Type | Article | |
| DOI | 10.3389/fmicb.2023.1175679 | |
| Abstract | Introduction: Infection with human T cell lymphotropic virus type 1 (HTLV-1) is endemic in Brazil and is linked with pro-inflammatory conditions including HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), a chronic neuroinflammatory incapacitating disease that culminates in loss of motor functions. The mechanisms underlying the onset and progression of HAM/TSP are incompletely understood. Previous studies have demonstrated that inflammation and infectious agents can affect the expression of cellular prion protein (PrPC) in immune cells. Methods: Here, we investigated whether HTLV-1 infection affected PrPC content in cell lines and primary CD4+cells in vitro using flow cytometry and western blot assays. Results: We found that HTLV-1 infection decreased the expression levels of PrPC and HTLV-1 Orf I encoded p12, an endoplasmic reticulum resident protein also known to affect post-transcriptionally cellular proteins such as MHC-class I and the IL-2 receptor. In addition, we observed a reduced percentage of CD4+ T cells from infected individuals expressing PrPC, which was reflected by IFN type II but not IL-17 expression. Discussion: These results suggested that PrPC downregulation, linked to both HTLV-1 p12 and IFN-γ expression in CD4+ cells, may play a role in the neuropathogenesis of HTLV-1 infection. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Imunologia Básica e Aplicada, Rio de Janeiro, RJ, Brasil / National Cancer Institute. Animal Models and Retroviral Vaccines Section. Vaccine Branch. Bethesda, United States. | en_US |
| Affilliation | Hospital for Sick Children. Program in Genetics and Genome Biology. Toronto, Canada. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurogenesis. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | National Cancer Institute. Vaccine Branch. Animal Models and Retroviral Vaccines Section. Bethesda, United States. | en_US |
| Affilliation | National Cancer Institute. Vaccine Branch. Animal Models and Retroviral Vaccines Section. Bethesda, United States. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurogenesis. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Imunologia Básica e Aplicada. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | HTLV-1 | en_US |
| Subject | HTLV-1-infected cells | en_US |
| Subject | IFNγ | en_US |
| Subject | PrPC | en_US |
| Subject | lymphocytes | en_US |
| Subject | p12 | en_US |
| e-ISSN | 1664-302X | |
