| Author | Bastidas, Juliana Girón | |
| Author | Maurmann, Natasha | |
| Author | Scholl, Juliete Nathali | |
| Author | Weber, Augusto Ferreira | |
| Author | Silveira, Raíssa Padilha | |
| Author | Figueiró, Fabricio | |
| Author | Stimamiglio, Marco Augusto | |
| Author | Marcon, Bruna | |
| Author | Correa, Alejandro | |
| Author | Pranke, Patricia | |
| Access date | 2024-01-16T14:55:45Z | |
| Available date | 2024-01-16T14:55:45Z | |
| Document date | 2023 | |
| Citation | BASTIDAS, Juliana Girón et al. Secretome of stem cells from human exfoliated deciduous teeth (SHED) and its extracellular vesicles improves keratinocytes migration, viability, and attenuation of H2O2- induced cytotoxicity. Polymers, v.31, p. 827-841, 2023. | en_US |
| ISSN | 1524-475X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/62201 | |
| Language | por | en_US |
| Publisher | Wiley | en_US |
| Rights | open access | en_US |
| Title | Secretome of stem cells from human exfoliated deciduous teeth (SHED) and its extracellular vesicles improves keratinocytes migration, viability, and attenuation of H2O2- induced cytotoxicity | en_US |
| Type | Article | en_US |
| DOI | https://doi.org/10.1111/wrr.13131 | |
| Abstract | Therapies for wound healing using the secretome and extracellular vesicles (EVs) of mesenchymal stem/stromal cells have been shown to be successful in preclinical studies. This study aimed to characterise the protein content of the secretome from stem cells from human exfoliated deciduous teeth (SHED) and analyse the in vitro effects of SHED-conditioned medium (SHED-CM) and SHED extracellular vesicles (SHED-EVs) on keratinocytes. EVs were isolated and characterised. The keratinocyte viability and migration of cells treated with SHED-EVs and conditioned medium (CM) were evaluated. An HaCaT apoptosis model induced by H2O2 in vitro was performed with H2O2 followed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) and live/dead assays. Finally, the expression of vascular endothelial growth factor (VEGF) in keratinocytes treated with secretome and EVs was evaluated by immunofluorescence staining and confirmed with RT-qPCR. SHED-EVs revealed a cup-shaped morphology with expression of the classical markers for exosomes CD9 and CD63, and a diameter of 181 ± 87 nm. The internalisation of EVs by HaCaT cells was confirmed by fluorescence microscopy. Proteomic analysis identified that SHEDCM is enriched with proteins related to stress response and development, including cytokines (CXCL8, IL-6, CSF1, CCL2) and growth factors (IGF2, MYDGF, PDGF). The results also indicated that 50% CM and 0.4–0.6 μg/mL EVs were similarly efficient for improving keratinocyte viability, migration, and attenuation of H2O2-induced cytotoxicity. Additionally, expression of VEGF on keratinocytes increased when treated with SHED secretome and EVs. Furthermore, VEGF gene expression in keratinocytes increased significantly when treated with SHED secretome and EVs. Both SHED-CM and SHED-EVs may therefore be promising therapeutic tools for accelerating re-epithelialization in wound healing. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Laboratório de Células Tronco e Hematologia. Porto Alegre, RS, Brasil. / Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Ciências Biológicas: Fisiologia. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Laboratório de Células Tronco e Hematologia. Porto Alegre, RS, Brasil. / Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Ciências Biológicas: Fisiologia. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Ciências Biológicas: Bioquímica. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Ciências Biológicas: Bioquímica. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Laboratório de Células Tronco e Hematologia. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Ciências Biológicas: Bioquímica. Porto Alegre, RS, Brasil./ Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Laboratório de Células Tronco e Hematologia. Porto Alegre, RS, Brasil. / Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Ciências Biológicas: Fisiologia. Porto Alegre, RS, Brasil. / Instituto de Pesquisa com Células-Tronco. Porto Alegre, RS, Brasil. | en_US |
| Subject | Extracellular Vesicles | en_US |
| Subject | Keratinocytes | en_US |
| Subject | Mesenchymal Stem Cells | en_US |
| Subject | Secretome | en_US |
| Subject | Tissue Regeneration | en_US |
| Subject | Wound Healing | en_US |
| Subject in Spanish | Vesículas Extracelulares | en_US |
| Subject in Spanish | Queratinocitos | en_US |
| Subject in Spanish | Células Madre Mesenquimatosas | en_US |
| Subject in Spanish | Secretoma | en_US |
| Subject in Spanish | Regeneración Tisular | en_US |
| Subject in Spanish | Cicatrización de Heridas | en_US |
| Subject in French | Vésicules extracellulaires | en_US |
| Subject in French | Kératinocytes | en_US |
| Subject in French | Cellules souches mésenchymateuses | en_US |
| Subject in French | Sécrétome | en_US |
| Subject in French | Régénération tissulaire | en_US |
| Subject in French | Cicatrisation de plaie | en_US |
| DeCS | Vesículas Extracelulares | en_US |
| DeCS | Queratinócitos | en_US |
| DeCS | Células-Tronco Mesenquimais | en_US |
| DeCS | Secretoma | en_US |
| DeCS | Regeneração Tecidual | en_US |
| DeCS | Cicatrização | en_US |
