| Author | Diniz, Luiza Alves | |
| Author | Ferreira, Luiza de Almeida Queiroz | |
| Author | Ribeiro, Rafaela de Brito | |
| Author | Jesus, Sarah Luiza Galvão de | |
| Author | Anestino, Thales Augusto | |
| Author | Caldeira, Alisson Samuel Portes | |
| Author | Souto, Giovanna Ribeiro | |
| Author | Avelar, Gleide Fernandes de | |
| Author | Amaral, Flávio Almeida | |
| Author | Ferreira, Marcus Vinícius Lucas | |
| Author | Madeira, Mila Fernandes Moreira | |
| Author | Braga, Fernão Castro | |
| Author | Diniz, Ivana Márcia Alves | |
| Access date | 2024-01-17T19:23:09Z | |
| Available date | 2024-01-17T19:23:09Z | |
| Document date | 2023 | |
| Citation | DINIZ, Luiza Alves et al. Exploring the association between a standardized extract of pequi peels (Caryocar brasiliense Cambess) and blue light as a photodynamic therapy for treating superficial wounds. Photochem Photobiol., 2023. doi: 10.1111/php.13874. | en_US |
| ISSN | 0031-8655 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/62267 | |
| Language | eng | en_US |
| Publisher | American Society for Photobiology | en_US |
| Rights | restricted access | |
| Title | Exploring the association between a standardized extract of pequi peels (Caryocar brasiliense Cambess) and blue light as a photodynamic therapy for treating superficial wounds | en_US |
| Type | Article | |
| Abstract | Natural products derived from plants can be used as photosensitizers for antimicrobial photodynamic therapy (aPDT) combining key therapeutic strategies for tissue repair while controlling microorganisms' growth. We investigated a standardized extract of pequi peels (Caryocar brasiliense Cambess) as a brownish natural photosensitizer for aPDT using blue light. Three concentrations of the pequi extract (PE; 10, 30, or 90 μg/mL) were tested solely or associated with blue laser (445 nm, 100 mW, 138 J/cm2 , 6 J, 60 s). In vitro, we quantified reactive oxygen species (ROS), assessed skin keratinocytes (HaCat) viability and migration, and aPDT antimicrobial activity on Streptococcus or Staphylococcus strains. In vivo, we assessed wound closure for the most active concentration disclosed by the in vitro assay (30 μg/mL). Upon aPDT treatments, ROS were significantly increased in cell monolayers regardless of PE concentration. PE at low doses stimulates epithelial cells. Although PE stimulated cellular migration, aPDT was moderately cytotoxic to skin keratinocytes, particularly at the highest concentration. The antimicrobial activity was observed for PE at the lowest concentration (10 μg/mL) and mostly at PE 10 μg/mL and 30 μg/mL when used as aPDT photosensitizers. aPDT with PE 30 μg/mL presents antimicrobial activity without compromising the initial phases of skin repair. | en_US |
| Affilliation | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/LASER Biotechnologies. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/LASER Biotechnologies. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Microbiology. Biological Sciences Institute. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Pharmaceutical Sciences. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/Vice Directorate of Research. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | LASER Biotechnologies. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/Department of Dentistry. Pontifícia Universidade Católica de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Morphology. Biological Sciences Institute. Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. | en_US |
| Affilliation | Department of Biochemistry and Immunology, Biological Sciences Institute. Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. | en_US |
| Affilliation | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Microbiology. Biological Sciences Institute. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Pharmaceutical Sciences. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/LASER Biotechnologies. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Staphylococcus aureus | en_US |
| Subject | Streptococcus mutans | en_US |
| Subject | blue light | en_US |
| Subject | keratinocytes | en_US |
| Subject | pequi fruit peels | en_US |
| Subject | photodynamic therapy | en_US |
| Subject | wound repair | en_US |
| Embargo date | 3100-12-31 | |
