| Author | Fernandez-Becerra, Carmen | |
| Author | Xander, Patrícia | |
| Author | Alfandari, Daniel | |
| Author | Dong, George | |
| Author | Aparici-Herraiz, Iris | |
| Author | Rosenhek-Goldian, Irit | |
| Author | Shokouhy, Mehrdad | |
| Author | Gualdron-Lopez, Melisa | |
| Author | Lozano, Nicholy | |
| Author | Cortes-Serra, Nuria | |
| Author | Abou Karam, Paula | |
| Author | Meneghetti, Paula | |
| Author | Madeira, Rafael Pedro | |
| Author | Porat, Ziv | |
| Author | Soares, Rodrigo Pedro Pinto | |
| Author | Costa, Adriana Oliveira | |
| Author | Rafati, Sima | |
| Author | ilva, Anabela-Cordeiro da S | |
| Author | Santarém, Nuno | |
| Author | Fernandez-Prada, Christopher | |
| Author | Ramirez, Marcel I. | |
| Author | Bernal, Dolores | |
| Author | Marcilla, Antonio | |
| Author | Pereira-Chioccola, Vera Lucia | |
| Author | Alves, Lysangela Ronalte | |
| Author | Portillo, Hernando Del | |
| Author | Regev-Rudzki, Neta | |
| Author | Almeida, Igor Correia de | |
| Author | Schenkman, Sergio | |
| Author | Olivier, Martin | |
| Author | Torrecilhas, Ana Claudia | |
| Access date | 2024-01-22T19:51:36Z | |
| Available date | 2024-01-22T19:51:36Z | |
| Document date | 2023 | |
| Citation | FERNANDEZ-BECERRA, Carmen et al. Guidelines for the purification and characterization of extracellularvesicles of parasites. J of Extracellular Bio., v. 2, n.10, e117, 2023. https://doi.org/10.1002/jex2.117 | en_US |
| ISSN | 2768-2811 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/62327 | |
| Language | eng | en_US |
| Publisher | Willey | en_US |
| Rights | restricted access | |
| Title | Guidelines for the purification and characterization of extracellular vesicles of parasites | en_US |
| Type | Article | |
| Abstract | Parasites are responsible for the most neglected tropical diseases, affecting over a billion people worldwide (WHO, 2015) and accounting for billions of cases a year and responsible for several millions of deaths. Research on extracellular vesicles (EVs) has increased in recent years and demonstrated that EVs shed by pathogenic parasites interact with host cells playing an important role in the parasite's survival, such as facilitation of infection, immunomodulation, parasite adaptation to the host environment and the transfer of drug resistance factors. Thus, EVs released by parasites mediate parasite-parasite and parasite-host intercellular communication. In addition, they are being explored as biomarkers of asymptomatic infections and disease prognosis after drug treatment. However, most current protocols used for the isolation, size determination, quantification and characterization of molecular cargo of EVs lack greater rigor, standardization, and adequate quality controls to certify the enrichment or purity of the ensuing bioproducts. We are now initiating major guidelines based on the evolution of collective knowledge in recent years. The main points covered in this position paper are methods for the isolation and molecular characterization of EVs obtained from parasite-infected cell cultures, experimental animals, and patients. The guideline also includes a discussion of suggested protocols and functional assays in host cells | en_US |
| Affilliation | ISGlobal, Barcelona Institute for Global Health. Hospital Clínic-Universitat de Barcelona. Barcelona, Spain/IGTP Institut d’Investigació Germans Trias i Pujol. Badalona. Spain/CIBERINFEC, ISCIII-CIBER de Enfermedades Infecciosas. Instituto de Salud Carlos III. Madrid, Spain | en_US |
| Affilliation | Universidade Federal de São Paulo. Instituto de Ciências Ambientais, Químicas e Farmacêuticas. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, Brazil | en_US |
| Affilliation | Department of Biomolecular Sciences. Weizmann Institute of Science. Rehovot, Israel | en_US |
| Affilliation | The Research Institute of the McGill University Health Centre. McGill University. Montréal, Québec, Canada | en_US |
| Affilliation | ISGlobal, Barcelona Institute for Global Health. Hospital Clínic-Universitat de Barcelona. Barcelona, Spain | en_US |
| Affilliation | ISGlobal, Barcelona Institute for Global Health. Hospital Clínic-Universitat de Barcelona. Barcelona, Spain | en_US |
| Affilliation | Department of Immunotherapy and Leishmania Vaccine Research. Pasteur Institute of Iran. Tehran, Iran | en_US |
| Affilliation | ISGlobal, Barcelona Institute for Global Health. Hospital Clínic-Universitat de Barcelona. Barcelona, Spain | en_US |
| Affilliation | Universidade Federal de São Paulo. Instituto de Ciências Ambientais, Químicas e Farmacêuticas. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, Brazil | en_US |
| Affilliation | ISGlobal, Barcelona Institute for Global Health. Hospital Clínic-Universitat de Barcelona. Barcelona, Spain | en_US |
| Affilliation | Department of Biomolecular Sciences. Weizmann Institute of Science. Rehovot, Israel | en_US |
| Affilliation | Universidade Federal de São Paulo. Instituto de Ciências Ambientais, Químicas e Farmacêuticas. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, Brazil | en_US |
| Affilliation | Universidade Federal de São Paulo. Instituto de Ciências Ambientais, Químicas e Farmacêuticas. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, Brazil | en_US |
| Affilliation | Flow Cytometry Unit. Life Sciences Core Facilities. WIS. Rehovot, Israel | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Department of Immunotherapy and Leishmania Vaccine Research. Pasteur Institute of Iran. Tehran, Iran | en_US |
| Affilliation | Host-Parasite Interactions Group. Institute of Research and Innovation in Health, University of Porto. Porto, Portugal/ Department of Biological Sciences. Faculty of Pharmacy. University of Porto. Porto, Portugal | en_US |
| Affilliation | Host-Parasite Interactions Group. Institute of Research and Innovation in Health, University of Porto. Porto, Portugal/ Department of Biological Sciences. Faculty of Pharmacy. University of Porto. Porto, Portugal | en_US |
| Affilliation | Faculty of Veterinary Medicine. Université de Montréal. Québec, Canada | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil Laboratório de Biologia Molecular e Sistemática de Tripanossomatideos. Extracellular Vesicles and Host-Parasite Interactions Research Group. | en_US |
| Affilliation | Departament de Bioquímica i Biologia Molecular. Facultat de Ciències Biològiques. Universitat de València. Burjassot, Valencia, Spain | en_US |
| Affilliation | Àrea de Parasitologia. Departament de Farmàcia i Tecnologia Farmacèutica i Parasitologia. Universitat de València. Burjassot, Valencia, Spain | en_US |
| Affilliation | Instituto Adolfo Lutz. Centro de Parasitologia e Micologia Laboratório de Biologia Molecular de Parasitas e Fungos. São Paulo, SP, Brasil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brazil/Center in Infectious Diseases. Division of Infectious Disease and Immunity CHU de Quebec Research Center. Department of Microbiology, Infectious Disease and Immunology. Faculty of Medicine. University Laval. Quebec City, Quebec, Canada | en_US |
| Affilliation | ISGlobal, Barcelona Institute for Global Health. Hospital Clínic-Universitat de Barcelona. Barcelona, Spain/ IGTP Institut d’Investigació Germans Trias i Pujol. Badalona. Spain/ICREA Institució Catalana de Recerca i Estudis Avanc ̧ats. Barcelona, Spain | en_US |
| Affilliation | Department of Biomolecular Sciences. Weizmann Institute of Science. Rehovot, Israel | en_US |
| Affilliation | Department of Biological Sciences. Border Biomedical Research Center. The University of Texas at El Paso. El Paso, TX, USA | en_US |
| Affilliation | Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | en_US |
| Affilliation | The Research Institute of the McGill University Health Centre. McGill University. Montréal, Québec, Canada | en_US |
| Affilliation | Universidade Federal de São Paulo. Instituto de Ciências Ambientais, Químicas e Farmacêuticas. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, Brazil | en_US |
| Subject | EVs methodology | en_US |
| Subject | extracellular vesicles | en_US |
| Subject | helminth | en_US |
| Subject | host-parasite interaction | en_US |
| Subject | infection | en_US |
| Subject | protocols | en_US |
| Subject | protozoan parasites | en_US |
| Embargo date | 3100-12-31 | |
