| Author | Guimarães, Lays Cordeiro | |
| Author | Costa, Pedro Augusto Carvalho | |
| Author | Scalzo Júnior, Sérgio Ricardo Aluotto | |
| Author | Ferreira, Heloísa Athaydes Seabra | |
| Author | Braga, Ana Carolina Soares | |
| Author | Oliveira, Leonardo Camilo de | |
| Author | Figueiredo, Maria Marta | |
| Author | Shepherd, Sarah | |
| Author | Hamilton, Alex | |
| Author | Queiroz-Junior, Celso Martins | |
| Author | Silva, Walison Nunes da | |
| Author | Silva, Natália Jordana Alves da | |
| Author | Alves, Marco Túllio Rodrigues | |
| Author | Santos, Anderson Kenedy | |
| Author | Faria, Kevin Kelton Santos de | |
| Author | Marim, Fernanda Martins | |
| Author | Fukumasu, Heidge | |
| Author | Birbrair, Alexander | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Aguiar, Renato Santana de | |
| Author | Mitchell, Michael J. | |
| Author | Teixeira, Mauro Martins | |
| Author | Costa, Vivian Vasconcelos | |
| Author | Frezard, Frederic | |
| Author | Guimaraes, Pedro Pires Goulart | |
| Access date | 2024-02-20T15:24:51Z | |
| Available date | 2024-02-20T15:24:51Z | |
| Document date | 2024 | |
| Citation | GUIMARÃES, Lays Cordeiro et al. Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models. Nature Communications, v. 15, n. 1, p. 1-19, Jan. 2024. | en_US |
| ISSN | 2041-1723 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/62681 | |
| Language | eng | en_US |
| Publisher | Nature Pub. Group | en_US |
| Rights | restricted access | |
| Title | Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models | en_US |
| Type | Article | |
| DOI | 10.1038/s41467-024-44830-1 | |
| Abstract | A safe and effective vaccine with long-term protection against SARS-CoV-2 variants of concern (VOCs) is a global health priority. Here, we develop lipid nanoparticles (LNPs) to provide safe and effective delivery of plasmid DNA (pDNA) and show protection against VOCs in female small animal models. Using a library of LNPs encapsulating unique barcoded DNA (b-DNA), we screen for b-DNA delivery after intramuscular administration. The top-performing LNPs are further tested for their capacity of pDNA uptake in antigen-presenting cells in vitro. The lead LNP is used to encapsulate pDNA encoding the HexaPro version of SARS-CoV-2 spike (LNP-HPS) and immunogenicity and protection is tested in vivo. LNP-HPS elicit a robust protective effect against SARS-CoV-2 Gamma (P.1), correlating with reduced lethality, decreased viral load in the lungs and reduced lung damage. LNP-HPS induce potent humoral and T cell responses against P.1, and generate high levels of neutralizing antibodies against P.1 and Omicron (B.1.1.529). Our findings indicate that the protective efficacy and immunogenicity elicited by LNP-HPS are comparable to those achieved by the approved COVID-19 vaccine from Biontech/Pfizer in animal models. Together, these findings suggest that LNP-HPS hold great promise as a vaccine candidate against VOCs. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Biochemistry and Immunology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | State University of Minas Gerais. Divinopolis, MG, Brazil. | en_US |
| Affilliation | Department of Bioengineering. University of Pennsylvania. Philadelphia, PA, USA. | en_US |
| Affilliation | Department of Bioengineering. University of Pennsylvania. Philadelphia, PA, USA. | en_US |
| Affilliation | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Genetics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Animal Science and Food Engineering. University of São Paulo. Pirassununga, SP, Brazil. | en_US |
| Affilliation | Department of Dermatology. University of Wisconsin-Madison. Madison, WI, USA. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Genetics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Bioengineering. University of Pennsylvania. Philadelphia, PA, USA. | en_US |
| Affilliation | Department of Biochemistry and Immunology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Animals | en_US |
| Subject | COVID-19 | en_US |
| Subject | DNA B-Form | en_US |
| Subject | Nanovaccines | en_US |
| Subject | SARS-CoV-2 | en_US |
| Subject | Vaccines DNA | en_US |
| Embargo date | 2030-12-31 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
