Author | Cestaro, Beatriz Isabella | |
Author | Machado, Kelly Cavalcanti | |
Author | Batista, Michel | |
Author | Silva, Bruno José Gonçalves da | |
Access date | 2024-02-27T14:41:59Z | |
Available date | 2024-02-27T14:41:59Z | |
Document date | 2024 | |
Citation | CESTARO, Beatriz Isabella et al. Hollow-fiber liquid phase microextraction for determination of fluoxetine in human serum by nano-liquid chromatography coupled to high resolution mass spectrometry. Journal of Chromatography B, v. 1234, p. 1-8, 2024. | en_US |
ISSN | 1570-0232 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/62808 | |
Language | por | en_US |
Publisher | Elsevier | en_US |
Rights | open access | en_US |
Subject in Portuguese | Soro humano | en_US |
Title | Hollow-fiber liquid phase microextraction for determination of fluoxetine in human serum by nano-liquid chromatography coupled to high resolution mass spectrometry | en_US |
Type | Article | en_US |
DOI | https://doi.org/10.1016/j.jchromb.2024.124018 | |
Abstract | Therapeutic drug monitoring (TDM) is a personalized care tool based on the determination of a target drug concentration in human serum. An antidepressant drug of interest for such investigations is fluoxetine (FXT), due to a severe impact of genetic polymorphisms on its metabolism. A bioanalytical method employed for TDM purposes must exhibit satisfactory selectivity and detectability, which becomes more difficult due to highly complex biological matrices. In this study, a highly selective bioanalytical method for the determination of FXT in human serum is proposed, which provides excellent clean-up efficiency based on a low cost hollow fiber liquid-phase microextraction (HF-LPME) sample preparation step and nano-liquid chromatography coupled to high-resolution mass spectrometry (nano-LC-HRMS). HF-LPME was performed using a two-phase “U” configuration, with 6 cm fiber, 20 μL of 1-octanol acting as supported liquid membrane, and ammonium hydroxide (pH 10) as the donor phase with NaCl (10 % m/v) and methanol (5 % v/v) as additives, requiring only 250 μL of the sample. The procedure was conducted for 30 min under a 750 rpm stirring rate. Gradient elution was carried out employing an acetonitrile–water as a mobile phase, the composition of 30:70 to 100:00 (v/v) for 15 min, using formic acid 0.1 % (v/v) as an additive. MS1 was acquired in an Orbitrap mass analyzer, while MS2 was acquired in a linear trap quadrupole. Satisfactory linearity (Pearson’s r = 0.99709) was obtained for a concentration range of 0.02 to 2.5 μg mL 1, which is compatible with the therapeutic and toxic range for FXT. The developed method presents adequate precision (1.61 to 7.45 %) and accuracy (95 to 114 %) and allows the dilution of high concentration samples in a 1:4 ratio (v/v), enabling its application for forensic serum samples. To our knowledge, this is the first study reporting a method based on HF-LPME and nano-LC-HRMS with any analytical purpose, especially with a TDM focus. | en_US |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Mass Spectrometry Facility - RPT02H. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Mass Spectrometry Facility - RPT02H. Curitiba, PR, Brasil. | en_US |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Curitiba, PR, Brasil. | en_US |
Subject | Human serum | en_US |
Subject | Fluoxetine | en_US |
Subject | Drug Monitoring | en_US |
Subject | Mass Spectrometry | en_US |
Subject | Chromatography, Liquid | en_US |
Subject in Spanish | Fluoxetina | en_US |
Subject in Spanish | Monitoreo de Drogas | en_US |
Subject in Spanish | Espectrometría de Masas | en_US |
Subject in Spanish | Cromatografía Liquida | en_US |
Subject in French | Fluoxétine | en_US |
Subject in French | Surveillance des médicaments | en_US |
Subject in French | Spectrométrie de masse | en_US |
Subject in French | Chromatographie en phase liquide | en_US |
DeCS | Fluoxetina | en_US |
DeCS | Monitoramento de Medicamentos | en_US |
DeCS | Espectrometria de Massas | en_US |
DeCS | Cromatografia Líquida | en_US |