| Author | Zomer, Helena Debiazi | |
| Author | Lima, Victor Juan de Souza | |
| Author | Bion, Monique Coelho | |
| Author | Brito, Karynne Nazare Lins | |
| Author | Rode, Michele | |
| Author | Stimamiglio, Marco Augusto | |
| Author | Jeremias, Talita da Silva | |
| Author | Trentin, Andrea Gonçalves | |
| Access date | 2024-03-07T14:37:17Z | |
| Available date | 2024-03-07T14:37:17Z | |
| Document date | 2024 | |
| Citation | ZOMER, Helena Debiazi et al. Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as effective as cells. Stem Cell Research & Therapy, v. 15, n. 15, p. 1–14, 2024. | en_US |
| ISSN | 1757-6512 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/62963 | |
| Language | por | en_US |
| Publisher | BMC | en_US |
| Rights | open access | en_US |
| Title | Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as effective as cells | en_US |
| Type | Article | en_US |
| DOI | https://doi.org/10.1186/s13287-023-03630-y | |
| Abstract | Although the paracrine effects of mesenchymal stem/stromal cells (MSCs) have been recognized as crucial mediators of their regenerative effects on tissue repair, the potential of MSC secretomes as effective substitutes for cellular therapies remains underexplored. In this study, we compared MSCs from the human dermis (DSCs) and adipose tissue (ASCs) with their secretomes regarding their efficacy for skin wound healing using a translationally relevant murine model. Proteomic analysis revealed that while there was a substantial overlap in protein composition between DSC and ASC secretomes, specific proteins associated with wound healing and angiogenesis were differentially expressed. Despite a similar angiogenic potential in vivo, DSC and ASC secretomes were found to be less effective than cells in accelerating wound closure and promoting tissue remodeling. Overall, secretome-treated groups showed intermediary results between cells- and control-treated (empty scaffold) groups. These findings highlight that although secretomes possess therapeutic potential, their efficacy might be limited compared to cellular therapies. This study contributes to the growing understanding of MSC secretomes, emphasizes the need for further protocol optimization, and offers insights into their potential applications in regenerative medicine. | en_US |
| Affilliation | Department of Physiological Sciences. University of Florida. Gainesville, USA. / Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. / Universidade Federal do Rio de Janeiro. Instituto Nacional de Neurociência Translacional. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. / Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Mesenchymal Stem Cells | en_US |
| Subject | Mesenchymal Stromal Cells | en_US |
| Subject | Dermis | en_US |
| Subject | Culture Media, Conditioned | en_US |
| Subject | Exosomes | en_US |
| Subject | Proteomics | en_US |
| Subject in Spanish | Células Madre Mesenquimatosas | en_US |
| Subject in Spanish | Medios de Cultivo Condicionados | en_US |
| Subject in Spanish | Exosomas | en_US |
| Subject in Spanish | Proteómica | en_US |
| Subject in French | Cellules souches mésenchymateuses | en_US |
| Subject in French | Milieux de culture conditionnés | en_US |
| Subject in French | Protéomique | en_US |
| DeCS | Células-Tronco Mesenquimais | en_US |
| DeCS | Células Estromais Mesenquimais | en_US |
| DeCS | Derme | en_US |
| DeCS | Meios de Cultivo Condicionados | en_US |
| DeCS | Exossomos | en_US |
| DeCS | Proteômica | en_US |
