| Author | Cruz, Aline C. | |
| Author | Neves, Bianca C | |
| Author | Higa, Laurinda Yoko Shinzato | |
| Author | Folescu, Tânia Wrobel | |
| Author | Marques, Elizabeth A. | |
| Author | Milagres, Lucimar G. | |
| Access date | 2013-02-05T14:03:06Z | |
| Available date | 2013-02-05T14:03:06Z | |
| Document date | 2012 | |
| Citation | CRUZ, Aline C. et al. Type III apparatus of Pseudomonas aeruginosa as a tool to diagnose pulmonary infection in cystic fibrosis patients. APMIS acta pathol. microbiol. immunol. scand., Copenhagen, v. 120, n. 8, p. 622-627, aug. 2012 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/6296 | |
| Language | eng | pt_BR |
| Publisher | Munksgaard | pt_BR |
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| Rights | restricted access | |
| Title | Type III apparatus of Pseudomonas aeruginosa as a tool to diagnose pulmonary infection in cystic fibrosis patients | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1600-0463.2012.02888.x | |
| Abstract | Pseudomonas aeruginosa is associated with increased mortality in cystic fibrosis (CF) patients, and expresses type III secretion system proteins (TTSP), which is a common mechanism used by gram-negative pathogens for delivery of anti-host factors. Our aim was to investigate whether or not these antigens (TTSP) would be recognized by CF sera, by Western blot reaction. We have showed herein that all patients (n = 11) not chronically infected by P. aeruginosa had their first serum positive for TTSP (ExoS, ExoT, PopB, and/or PopD). All chronic patients had a strong positive serology to TTSP, although relatively weak reactions to TTSP were observed for some individuals in the negative control group. Therefore, TTSP that were early produced in P. aeruginosa infected CF patients, induced a detectable antibody response in those patients and were easily detected by Western-blot reaction. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Deparamento de Bioquímica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pulmonologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pulmonologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Antibody Response | pt_BR |
| Subject | P. Aeruginosa | pt_BR |
| Subject | Pulmonary Infection | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Cystic Fibrosis | pt_BR |
| DeCS | Infecções por Pseudomonas | pt_BR |
| DeCS | Pseudomonas aeruginosa | pt_BR |
| DeCS | Infecções Respiratórias | pt_BR |
| DeCS | Fibrose Cística | pt_BR |
