| Author | Miranda, Rebeca Vitória da Silva Lage de | |
| Author | Monteiro, Giovanna Merrelho | |
| Author | Costa, Luciana Veloso da | |
| Author | Santos, Milena Cristina Silva dos | |
| Author | Reis, Cristhiane Moura Falavina dos | |
| Author | Braga, Lygia Maria Paulo da Silva | |
| Author | Forsythe, Stephen James | |
| Author | Bôas, Maria Helena Simões Villas | |
| Author | Brandão, Marcelo Luiz Lima | |
| Access date | 2024-03-12T19:40:50Z | |
| Available date | 2024-03-12T19:40:50Z | |
| Document date | 2023 | |
| Citation | MIRANDA, Rebeca Vitória da Silva Lage de et al. Evaluation of phenotypical and genotypical methods for the identification and typing of Stenotrophomonas maltophilia isolated from a pharmaceutical facility. Journal of Applied Microbiology, v. 134, n. 10, p. 1-12, Oct. 2023. | en_US |
| ISSN | 1364-5072 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/63026 | |
| Language | eng | en_US |
| Publisher | Oxford University Press | |
| Rights | restricted access | |
| Subject in Portuguese | Stenotrophomonas maltophilia | en_US |
| Subject in Portuguese | Instalação farmacêutica | en_US |
| Subject in Portuguese | Caracterização fenotípica | en_US |
| Subject in Portuguese | ERIC-PCR | en_US |
| Subject in Portuguese | MALDI TOF-MS | en_US |
| Subject in Portuguese | Antibiograma | en_US |
| Title | Evaluation of phenotypical and genotypical methods for the identification and typing of Stenotrophomonas maltophilia isolated from a pharmaceutical facility | en_US |
| Type | Article | |
| DOI | 10.1093/jambio/lxad236 | |
| Abstract | Aims: Evaluate methods for identification and typing of Stenotrophomonas maltophilia isolated from a pharmaceutical facility. Methods and results: From 270 S. maltophilia strains identified by VITEK ®2, 40 were selected and submitted to MALDI TOF-MS, 16S and 23S rRNA gene analysis, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and an antimicrobial susceptibility profile. 16S rRNA sequencing was able to identify 39 (97.5%) strains as Stenotrophomonas spp. and one (2.5%) as Luteimonas huabeiensis . MALDI TOF-MS identified 37 (92.5%) strains as S. maltophilia , and three (7.5%) were not identified. PCR targeting 23S rRNA yielded a positive result for 39 (97.5%) strains. However, after sequencing , t wo strains were identified as Stenotrophomonas rhizophila , showing false-positive re- sults. The confirmed S. maltophilia strains ( n = 37) showed 35 distinct ERIC-PCR profiles and exhibited sensitivity to minocycline and levofloxacin, and six (16.3%) sho w ed intermediate resistance to sulf ametho xaz ole-trimethoprim. Conclusion: Matrix-assisted laser desorption lonization-time of flight mass spectrometry (MALDI-TOF MS) was a satisfactory methodology for the identification of S. maltophilia , but expansion of the database is necessary for the identification of other species. 16S rDNA sequencing sho w ed lo w resolution f or Stenotrophomonas species differentiation. PCR targeting 23S rRNA could not differentiate S. maltophilia from S. rhizophila . ERIC-PCR was shown to be a useful tool for the microbial source tracking of S. maltophilia . Impact Statement Not all methodologies can correctly identify S. maltophilia strains isolated in pharmaceutical facility. In the present study, MALDI-TOF MS was the most reliable method for the identification of S. maltophilia . Studies that discuss identification and the resistance profile of Stenotrophomonas maltophilia isolated from pharmaceutical industry environ- ments are very scarce. This information is extremely relevant in understanding the contamination source and its persistence in se v eral samples of the production chain. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Controle de Qualidade. Laboratório de Controle Microbiológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Controle de Qualidade. Laboratório de Controle Microbiológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Controle de Qualidade. Laboratório de Controle Microbiológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Controle de Qualidade. Laboratório de Controle Microbiológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Controle de Qualidade. Laboratório de Controle Microbiológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Controle de Qualidade. Laboratório de Controle Microbiológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Foodmicrobe.com Ltd. Adams Hill, Keyworth, Nottingham, United Kingdom. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Alimentos e Saneantes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Controle de Qualidade. Laboratório de Controle Microbiológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Stenotrophomonas maltophilia | en_US |
| Subject | Pharmaceutical facility | en_US |
| Subject | Phenotypical characterization | en_US |
| Subject | ERIC-PCR | en_US |
| Subject | MALDI TOF-MS | en_US |
| Subject | Antibiogram | en_US |
| DeCS | Stenotrophomonas maltophilia | en_US |
| DeCS | Testes de Sensibilidade Microbiana | en_US |
| e-ISSN | 1365-2672 | |
| Embargo date | 2030-12-31 | |
