| Author | Camargo, Bruno Dias | |
| Author | Stracke, Mateus Cassaboni | |
| Author | Sanchuki, Heloisa Bruna Soligo | |
| Author | Oliveira, Viviane Klassen de | |
| Author | Ancelmo, Hellen Cristina | |
| Author | Bordin, Dayanne Mozaner | |
| Author | Marchini, Fabricio Klerynton | |
| Author | Viana, Emilson Ribeiro | |
| Author | Blanes, Lucas | |
| Access date | 2024-04-04T18:40:47Z | |
| Available date | 2024-04-04T18:40:47Z | |
| Document date | 2024 | |
| Citation | BIEMBENGUT, Ísis Venturi et al. Identification of CYFIP2 Arg87Cys ligands via in silico and in vitro approaches. Biomedicines, v. 12, n. 479, p. 1-15, 2024. | en_US |
| ISSN | 2079-6374 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/63404 | |
| Language | por | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Arduino | en_US |
| Subject in Portuguese | RT-LAMP | en_US |
| Title | Low-cost Arduino reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) for sensitive nucleic acid detection | en_US |
| Type | Article | en_US |
| DOI | https:// doi.org/10.3390/bios14030128 | |
| Abstract | This work presents a low-cost transcription loop-mediated isothermal amplification (RTLAMP) instrument for nucleic acid detection, employing an Arduino Nano microcontroller. The cooling system includes customized printed circuit boards (PCBs) that serve as electrical resistors and incorporate fans. An aluminum block is designed to accommodate eight vials. The system also includes two PCB heaters—one for sample heating and the other for vial lid heating to prevent condensation. The color detection system comprises a TCS3200 color 8-sensor array coupled to one side of the aluminum heater body and a white 8-LED array coupled to the other side, controlled by two Multiplexer/Demultiplexer devices. LED light passes through the sample, reaching the color sensor and conveying color information crucial for detection. The top board is maintained at 110 ± 2◦C, while the bottom board is held at 65 ± 0.5 ◦C throughout the RT-LAMP assay. Validation tests successfully demonstrated the efficacy of the colorimetric RT-LAMP reactions using SARS-CoV-2 RNA amplification as a sample viability test, achieving 100% sensitivity and 97.3% specificity with 66 clinical samples. Our instrument offers a cost-effective (USD 100) solution with automated result interpretation and superior sensitivity compared to visual inspection. While the prototype was tested with SARS-CoV-2 RNA samples, its versatility extends to detecting other pathogens using alternative primers, showcasing its potential for broader applications in biosensing. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | en_US |
| Affilliation | School of Mathematical and Physical Sciences. Faculty of Science. University of Technology Sydney. Ultimo, Australia. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Tecnológica Federal do Paraná. Departamento de Física. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | en_US |
| Subject | COVID-19 | en_US |
| Subject | Proof of Concept Study | en_US |
| Subject | Biosensing Techniques | en_US |
| Subject | Nucleic Acid Amplification Techniques | en_US |
| Subject in Spanish | Prueba de Ácido Nucleico para COVID-19 | en_US |
| Subject in Spanish | Prueba de Estudio Conceptual | en_US |
| Subject in Spanish | Técnicas Biosensibles | en_US |
| Subject in Spanish | Técnicas de Amplificación de Ácido Nucleico | en_US |
| Subject in French | Détection de l'acide nucléique du virus de la COVID-19 | en_US |
| Subject in French | Étude de validation de principe | en_US |
| Subject in French | Techniques de biocapteur | en_US |
| Subject in French | Techniques d'amplification d'acides nucléiques | en_US |
| DeCS | Teste de Ácido Nucleico | en_US |
| DeCS | Protótipo de Prova de Conceito | en_US |
| DeCS | Técnicas Biossensoriais | en_US |
| DeCS | Técnicas de Amplificação de Ácido Nucleico | en_US |
