| Author | Pavan, Tycha Bianca Sabaini | |
| Author | Leony, Leonardo Maia | |
| Author | Souza, Wayner Vieira de | |
| Author | Santos, Emily Ferreira | |
| Author | Daltro, Ramona Tavares | |
| Author | Freitas, Natália Erdens Maron | |
| Author | Vasconcelos, Larissa Carvalho Medrado | |
| Author | Habib, Fernanda Lopes | |
| Author | Silva, Ângelo Antônio Oliveira | |
| Author | Celedon, Paola Alejandra Fiorani | |
| Author | Sampaio, Daniel Dias | |
| Author | Zanchin, Nilson Ivo Tonin | |
| Author | Longhi, Silvia Andrea | |
| Author | Santos, Fred Luciano Neves | |
| Access date | 2024-04-24T15:41:44Z | |
| Available date | 2024-04-24T15:41:44Z | |
| Document date | 2024 | |
| Citation | PAVAN, Tycha Bianca Sabaini et al. Post-therapeutic cure criterion in chronic Chagas Disease using Trypanosoma cruzi chimeric proteins. Folia Parasitologica., v. 71, n. 4, p. 1-9, 2024. | en_US |
| ISSN | 1078-1439 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/63659 | |
| Language | por | en_US |
| Publisher | Institute of Parasitology | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Benznidazol | en_US |
| Subject in Portuguese | Nifurtimox | en_US |
| Title | Post-therapeutic cure criterion in chronic Chagas Disease using Trypanosoma cruzi chimeric proteins | en_US |
| Type | Article | en_US |
| DOI | 10.14411/fp.2024.004 | |
| Abstract | Chagas disease (CD) is a neglected disease caused by Trypanosoma cruzi Chagas, 1909. Causative treatment can be achieved with two drugs: benznidazole or Nifurtimox. There are some gaps that hinder progress in eradicating the disease. There is no test that can efficiently assess cure control after treatment. Currently, the decline in anti-T. cruzi antibody titres are assessed with conventional serological tests, which can take years. However, the search for new markers of cure must continue to fill this gap. The present study aimed to evaluate the decline in serological titre using chimeric proteins after treatment with benznidazole in chronic patients diagnosed with CD. It was a prospective cross-sectional cohort study between 2000 and 2004 of T. cruzi-positive participants from the Añatuya region (Argentina) treated with benznidazole. Serum samples from ten patients were collected before treatment (day zero) and after the end of treatment (2, 3, 6, 12, 24 and 36 months). For the detection of anti-T. cruzi antibodies, an indirect ELISA was performed using two chimeric recombinant proteins (IBMP-8.1 and IBMP-8.4) as antigens. The changes in reactivity index within the groups before and after treatment were evaluated using the Friedman test. All participants experienced a decrease in serological titres after treatment with benznidazole, especially IBMP-8.1. However, due to the small number of samples and the short follow-up period, it is premature to conclude that this molecule serves as a criterion for sustained cure. Further studies are needed to validate tests based on these or other biomarkers to demonstrate parasitological cure. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Saúde Pública. Recife, PE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanossomatídeos. Curitiba, PR, Brasil. | en_US |
| Affilliation | Prefeitura Municipal de Tremedal. Departamento de Saúde. Programa Brasileiro de Estratégia em Saúde da Família. Tremedal, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Laboratory of Molecular Biology of Chagas Disease. Institute for Research on Genetic Engineering and Molecular Biology “Dr Héctor Torres”. National Scientific and Technological Research Council. Buenos Aires, Argentina. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Saúde Pública Avançada. Salvador, BA, Brasil. / Fundação Oswaldo Cruz. Programa de Pesquisa Translacional em Doença de Chagas (Fio-Chagas). Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Chagas Disease | en_US |
| Subject | Benznidazole | en_US |
| Subject | Serologic Tests | en_US |
| Subject | Seroconversion | en_US |
| Subject | Antibody titre | en_US |
| Subject | Biomarkers | en_US |
| Subject in Spanish | Enfermedad de Chagas | en_US |
| Subject in Spanish | Pruebas Serológicas | en_US |
| Subject in Spanish | Seroconversión | en_US |
| Subject in French | Maladie de Chagas | en_US |
| Subject in French | Tests sérologiques | en_US |
| Subject in French | Séroconversion | en_US |
| Subject in French | Marqueurs biologiques | en_US |
| DeCS | Doença de Chagas | en_US |
| DeCS | Testes Sorológicos | en_US |
| DeCS | Soroconversão | en_US |
| DeCS | Biomarcadores | en_US |
