| Author | Espírito-Santo, Renan Fernandes do | |
| Author | Santos, Dourivaldo Silva | |
| Author | Lauria, Pedro Santana Sales | |
| Author | Lima, Alyne Almeida de | |
| Author | Abreu, Lucas Silva | |
| Author | Tavares, Josean Fechine | |
| Author | Castilho, Marcelo Santos | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Villarreal, Cristiane Flora | |
| Access date | 2024-04-25T12:17:08Z | |
| Available date | 2024-04-25T12:17:08Z | |
| Document date | 2023 | |
| Citation | ESPÍRITO-SANTO, Renan Fernandes do et al. Tonantzitlolone B modulates the endogenous opioid system to promote antinociception in mice. Journal of Natural Products, v. 86, n. 11, p. 1-8, Nov. 2023. | en_US |
| ISSN | 1520-6025 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/63689 | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). | en_US |
| Language | eng | en_US |
| Publisher | American Chemical Society | en_US |
| Rights | restricted access | |
| Subject in Portuguese | Tonantzitlolona B | en_US |
| Subject in Portuguese | Endógeno | en_US |
| Subject in Portuguese | Sistema opioide | en_US |
| Subject in Portuguese | Antinocicepção | en_US |
| Subject in Portuguese | Ratos | en_US |
| Title | Tonantzitlolone B modulates the endogenous opioid system to promote antinociception in mice | en_US |
| Type | Article | |
| DOI | 10.1021/acs.jnatprod.3c00731 | |
| Abstract | Tonantzitlolone B (TZL-B) is a diterpene isolated from the roots of Stillingia loranthacea. Its antinociceptive effects were investigated in male Swiss mice using the following models of pain: formalin test, inflammation induced by Complete Freund's Adjuvant (CFA), tail flick test, and cold plate test. The influence of TZL-B on the opioid system was assessed in vivo, using opioid antagonists; in silico, investigating the chemical similarity among TZL-B and opioid agonists; and ex vivo, measuring preproenkephalin (PENK) gene expression in the spinal cord by RT-qPCR. TZL-B (10-1000 μg/kg) promoted antinociception in the four experimental models without impairing mice's motor function. TZL-B did not alter paw edema during CFA-induced inflammation. The antinociceptive effects of TZL-B in the tail flick and cold plate tests were diminished by the opioid antagonists naloxone (5 mg/kg), NOR-BNI (0.5 mg/kg), naltrindole (3 mg/kg), and CTOP (1 mg/kg), indicating the involvement of κ-, δ-, and μ-opioid receptors. TZL-B showed no significant chemical similarity to opioid agonists, but the treatment with TZL-B (1000 μg/kg) increased PENK gene expression in the spinal cord of mice. These data suggest that TZL-B promotes antinociception by enhancing the transcription of PENK, hence modulating the endogenous opioid system. | en_US |
| Affilliation | Center to Advance Chronic Pain Research. University of Maryland. Baltimore, Maryland, USA. | en_US |
| Affilliation | Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal Fluminense. Instituto de Química. Niterói, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal da Paraíba. Instituto de Pesquisa sobre Drogas e Medicamentos. João Pessoa, PB, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Sistemas Avançados em Saúde. SENAI CIMATEC. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Subject | Tonantzitlolone B | en_US |
| Subject | Endogenous | en_US |
| Subject | Opioid system | en_US |
| Subject | Antinociception | en_US |
| Subject | Mice | en_US |
| Embargo date | 2030-12-31 | |
