Author | Silva, Ângelo Antônio Oliveira | |
Author | Lima, Ayla Araújo | |
Author | Vasconcelos, Larissa de Carvalho Medrado | |
Author | Almeida, Rosângela Andrade de | |
Author | Freitas, Natália Erdens Maron de | |
Author | Habib, Fernanda Lopes | |
Author | Oliva, Talita Andrade | |
Author | Silva, iralba Freire de Carvalho Ribeiro da | |
Author | Siqueira, Isadora Cristina de | |
Author | Santos, Fred Luciano Neves | |
Access date | 2024-04-30T12:25:04Z | |
Available date | 2024-04-30T12:25:04Z | |
Document date | 2024-04-09 | |
Citation | SILVA, Ângelo Antônio Oliveira et al. Performance assessment of treponemal and nontreponemal tests for the diagnosis of acquired syphilis. American Journal of Tropical Medicine and Hygiene, v. 00, n. 00, p. 1-8, 2024. | en_US |
ISSN | 1476-1645 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/63756 | |
Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior do Brasil (CAPES).
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
Conselho Nacional de Desenvolvimento Científico e Tecnológico Brasil (CNPq). | en_US |
Language | eng | en_US |
Publisher | American Society of Tropical Medicine and Hygiene | en_US |
Rights | restricted access | en_US |
Subject in Portuguese | Teste treponemo | en_US |
Subject in Portuguese | Testes não treponemo | en_US |
Subject in Portuguese | Diagnóstico | en_US |
Subject in Portuguese | Sífilis adquirida | en_US |
Title | Performance assessment of treponemal and nontreponemal tests for the diagnosis of acquired syphilis | en_US |
Type | Article | en_US |
DOI | 10.4269/ajtmh.23-0238 | |
Abstract | There are a variety of nontreponemal test (NTT) and treponemal test (TT) kits for the serologic diagnosis of syphilis. Because of the complexity of the infection (multiple clinical stages) and the different antigens used in these kits, a systematic evaluation of the accuracy of the currently available commercial tests is warranted. Our objective was to evaluate the performance of commercially available tests for the diagnosis of syphilis infection. In this study, we analyzed one NTT (Venereal Disease Research Laboratory [VDRL] test, Wiener Laboratories, Rosario, Argentina) and two TTs (fluorescent treponemal antibody absorption [FTA-ABS] test, Euroimmun, Lübeck, Germany, and syphilis recombinant ELISA v. 4.0 test [ELISA], Wiener Laboratories, Rosario, Argentina) using a panel of 187 samples, including serum samples from 31 individuals with primary syphilis, 77 with secondary syphilis, and 79 with latent syphilis. An additional 192 samples from uninfected individuals and 323 serum samples from individuals with other diseases were included. The sensitivities of the VDRL, ELISA, and FTA-ABS tests were 97.9%, 100%, and 96.3%, respectively. The VDRL and ELISA tests showed a specificity of 100%, and the FTA-ABS test showed a specificity of 99.5%. Accuracy was 98.9% for the VDRL test, 100% for the ELISA, and 97.9% for the FTA-ABS test. For primary, secondary, and latent syphilis, the ELISA achieved a diagnostic performance of 100%, whereas the sensitivity for the VDRL and FTA-ABS tests ranged from 96.8% to 98.7% and 93.7% to 98.7%, respectively. No difference was observed when the tests were used as traditional or reverse algorithms. In general, all three tests are able to discriminate positive and negative samples for syphilis, regardless of the diagnostic algorithm. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Universidade Salvador (UNIFACS). Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
Affilliation | Centro Estadual Especializado em Diagnóstico, Assistência e Pesquisa (CEDAP). Salvador, BA, Brasil. | en_US |
Affilliation | Centro Estadual Especializado em Diagnóstico, Assistência e Pesquisa (CEDAP). Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Programa Translacional Integrado em Doença de Chagas da Fiocruz. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Treponemal tests | en_US |
Subject | Nontreponemal tests | en_US |
Subject | Diagnosis | en_US |
Subject | Acquired syphilis | en_US |
DeCS | Treponema | en_US |
DeCS | Diagnóstico | en_US |
DeCS | Sífilis | en_US |
Embargo date | 2025-04-09 | |