Author | Nascimento, Leile Camila Jacob | |
Author | Portilho, Moyra, M. | |
Author | Anjos, Rosângela O. | |
Author | Moreira, Patrícia S. S. | |
Author | Stauber, Christine | |
Author | Weaver, Scott C. | |
Author | Kitron, Uriel | |
Author | Reis, Mitermayer G. | |
Author | Ribeiro, Guilherme S. | |
Access date | 2024-05-03T18:14:30Z | |
Available date | 2024-05-03T18:14:30Z | |
Document date | 2024-02-02 | |
Citation | NASCIMENTO, Leile Camila Jacob et al. Detection of chikungunya virus RNA in oral fluid and urine: an alternative approach to diagnosis? Viruses, v. 16, n. 2, p. 1-10, 2024. | en_US |
ISSN | 1999-4915 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/63863 | |
Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Ministério da Educação do Brasil.
Os Institutos Nacionais de Saúde.
Ministério da Saúde, Secretaria de Ciência, Tecnologia e Insumos Estratégicos. Departamento de Ciência e Tecnologia.
Fundação Oswaldo Cruz.
Universidade Federal da Bahia.
REPLICK (Pesquisa Clínica e Aplicada em Chikungunya). | en_US |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Subject in Portuguese | Chikungunya | en_US |
Subject in Portuguese | RT-qPCR | en_US |
Subject in Portuguese | Diagnóstico molecular | en_US |
Subject in Portuguese | Urina | en_US |
Subject in Portuguese | Fluido oral | en_US |
Title | Detection of chikungunya virus RNA in oral fluid and urine: an alternative approach to diagnosis? | en_US |
Type | Article | en_US |
DOI | 10.3390/ v16020235 | |
Abstract | To evaluate whether oral fluids (OF) and urine can serve as alternative, non-invasive samples to diagnose chikungunya virus (CHIKV) infection via RT-qPCR, we employed the same RNA extraction and RT-qPCR protocols on paired serum, OF and urine samples collected from 51 patients with chikungunya during the acute phase of the illness. Chikungunya patients were confirmed through RT-qPCR in acute-phase sera (N = 19), IgM seroconversion between acute- and convalescent-phase sera (N = 12), or IgM detection in acute-phase sera (N = 20). The controls included paired serum, OF and urine samples from patients with non-arbovirus acute febrile illness (N = 28) and RT-PCR-confirmed dengue (N = 16). Nine (47%) of the patients with positive RT-qPCR for CHIKV in sera and two (17%) of those with CHIKV infection confirmed solely via IgM seroconversion had OF positive for CHIKV in RT-qPCR. One (5%) patient with CHIKV infection confirmed via serum RT-qPCR was positive in the RT-qPCR performed on urine. None of the negative control group samples were positive. Although OF may serve as an alternative sample for diagnosing acute chikungunya in specific settings, a negative result cannot rule out an infection. Further research is needed to investigate whether OF and urine collected later in the disease course when serum becomes RT-qPCR-negative may be helpful in CHIKV diagnosis and surveillance, as well as to determine whether urine and OF pose any risk of CHIKV transmission. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | School of Public Health. Georgia State University. Atlanta, GA, USA. | en_US |
Affilliation | Department of Microbiology & Immunology and World Reference Center for Emerging Viruses and Arboviruses. University of Texas Medical Branch. Galveston, TX, USA. | en_US |
Affilliation | Department of Environmental Sciences. Emory University. Atlanta, GA, USA. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / School of Public Health. Georgia State University. Atlanta, GA, USA. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | en_US |
Subject | Chikungunya | en_US |
Subject | RT-qPCR | en_US |
Subject | Molecular diagnosis | en_US |
Subject | Urine | en_US |
Subject | Oral fluid | en_US |
DeCS | Vírus chikungunya | en_US |
DeCS | Técnicas de diagnóstico molecular | en_US |
DeCS | Urina | en_US |