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https://www.arca.fiocruz.br/handle/icict/6386
Tipo
ArtículoDerechos de autor
Acceso restringido
Fecha del embargo
2030-12-31
Colecciones
- IFF - Artigos de Periódicos [1282]
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CELLS ISOLATED FROM BONE-MARROW AND LUNGS OF ALLERGIC BALB/C MICE AND CULTURED IN THE PRESENCE OF IL-5 ARE RESPECTIVELY RESISTANT AND SUSCEPTIBLE TO APOPTOSIS INDUCED BY DEXAMETHASONE
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Institut Pasteur. Uniteé Associée INSERM. Unité de Pharmacologie Cellulaire. Paris, France.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Farmacologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Institut Pasteur. Uniteé Associée INSERM. Unité de Pharmacologie Cellulaire. Paris, France.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Farmacologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
We have previously reported that, in IL-5-stimulated bone-marrow cultures, dexamethasone upregulates eosinophil differentiation and protects developing eosinophils from apoptosis induced by a variety of agents. Recently developed procedures for the isolation of hemopoietic cells from allergic murine lungs have enabled us to evaluate how these cells respond to dexamethasone in IL-5-stimulated cultures, when compared with bone-marrow-derived cells isolated from the same donors, and whether differences in response patterns were linked to apoptosis. Ovalbumin challenge of sensitized mice increased significantly the numbers of mature leukocytes as well as hemopoietic cells recovered from digested lung fragments, relative to saline-challenged, sensitized controls. Both mature eosinophils and cells capable of differentiating into eosinophils in the presence of IL-5 were present in lungs from sensitized mice 24 h after airway challenge. Dexamethasone strongly inhibited eosinophil differentiation in IL-5-stimulated cultures of lung hemopoietic cells. By contrast, dexamethasone enhanced eosinophil differentiation in cultures of allergic bone-marrow cells, in identical conditions. Hemopoietic cells from lungs and bone-marrow were respectively susceptible and resistant to induction of apoptosis by dexamethasone. The dexamethasone-sensitive step was the response to IL-5 in culture, while accumulation of IL-5 responsive cells in allergen-challenged lungs was dexamethasone-resistant. Cells from lungs and bone-marrow, cultured for 3 days with IL-5 in the absence of dexamethasone, did not respond to a subsequent exposure to dexamethasone in the presence of IL-5. These findings confirm that IL-5-responsive hemopoietic cells found in challenged, sensitized murine lungs differ from those in bone-marrow, with respect to the cellular responses induced by dexamethasone, including apoptosis.
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