Author | Fontes, Silvia da Silva | |
Author | Conte, Fernando de Paiva | |
Author | Fernandes, Jorlan | |
Author | Oliveira, Renata Carvalho de | |
Author | Silva, Rodrigo Nunes Rodrigues da | |
Access date | 2024-05-06T16:39:00Z | |
Available date | 2024-05-06T16:39:00Z | |
Document date | 2024 | |
Citation | FONTES, Silvia da Silva et al. Design of a global multiepitope orthohantavirus vaccine: An Immunoinformatics Approach. In: INTERNATIONAL SYMPOSIUM ON IMMUNOBIOLOGICAL, 8., 2024. Rio de Janeiro. Annals... Rio de Janeiro: Bio-Manguinhos, 2024. p. 27. | en_US |
Citation | 10.35259/isi.biomang.2024_63899 | |
URI | https://www.arca.fiocruz.br/handle/icict/63899 | |
Language | por | en_US |
Publisher | Instituo de Tecnologia em Imunobiológicos | en_US |
Rights | open access | en_US |
Subject in Portuguese | Imunoterapia | en_US |
Subject in Portuguese | Bioinformática | en_US |
Subject in Portuguese | Orthohantavírus | en_US |
Subject in Portuguese | Epitopos | en_US |
Title | Design of a global multiepitope orthohantavirus vaccine: An Immunoinformatics Approach | en_US |
Type | Papers presented at events | en_US |
Abstract | Introduction: Hantaviruses, responsible for Hemorrhagic Fever with Renal Syndrome (HFRS) in Europe
and Asia, and Hantavirus Cardiopulmonary Syndrome (HCPS) in the Americas, pose a significant public
health challenge. Transmission occurs through inhalation of aerosols from infected rodent droppings, yet
no FDA- approved vaccine exists. The glycoprotein (GP) on the virion surface, crucial for host invasion
and highly immunogenic, is a primary vaccine target. However, GP variability among hantavirus species
presents a significant obstacle to vaccine development. Epitope-based vaccines offer a promising route to
a universal hantavirus vaccine.
Objectives: The objective is to leverage immunoinformatics to design a universal multi-epitope vaccine
that could be effective worldwide against both HFRS and HCPS.
Methodology: Using eight algorithms, GPs of SEOV and PUUV (HFRS) and SNV and ANDV (HCPS)
were analyzed to identify B cell epitopes. T cell epitopes were identified using the TepiTool algorithm.
These epitopes underwent allergenicity, toxicity, and hemotoxicity evaluations, along with conservation
analysis and population coverage assessment using the IEDB server. Two vaccine designs were then
proposed, incorporating different adjuvants (β-defensin and 50S ribosomal protein L7/L12), and analyzed
for physicochemical properties, antigenicity, and allergenicity. Tertiary structures were predicted, and TL4
affinity was assessed through molecular docking.
Results: Eleven sequences combining B and T cell epitopes, found to be non-allergenic, non-toxic, and
highly conserved among HFRS and HCPS hantaviruses, were selected for the vaccine composition.
Predicted to cover 100% of the global population, both vaccine designs showed promise in antigenicity,
stability, and solubility. Molecular docking demonstrated stable structures with a higher affinity for
β-defensin. Immunization simulations indicated an effective immune response and memory cell
persistence over a year.
Conclusion: This approach presents potential multiepitope vaccine candidates against hantavirus infection
diseases, demonstrating the utility of immunoinformatics in vaccine design. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Bioinformatics | en_US |
Subject | Hantavirus | en_US |
Subject | Epitopes | en_US |
Subject | Immunotherapy | en_US |
DeCS | Orthohantavírus | en_US |
DeCS | Epitopos | en_US |
DeCS | Imunoterapia | en_US |
DeCS | Biologia Computacional | en_US |