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| Autor | Macaubas, Juliana Pierin Jorge | |
| Autor | Omae, Samanta Camalhonte | |
| Autor | Guardalini, Luis Giovani Oliveira | |
| Autor | Bernardino, Thaissa Consoni | |
| Autor | Jorge, Soraia Attié Calil | |
| Autor | Quintilio, Wagner | |
| Fecha de acceso | 2024-05-07T13:01:05Z | |
| Fecha de disponibilización | 2024-05-07T13:01:05Z | |
| Fecha de publicación | 2024 | |
| Referencia | MACAUBAS, Juliana Pierin Jorge et al. Expression of the Rabies Virus Glycoprotein in vitro through three mRNA Delivery Systems. In: INTERNATIONAL SYMPOSIUM ON IMMUNOBIOLOGICAL, 8., 2024. Rio de Janeiro. Annals... Rio de Janeiro: Bio-Manguinhos, 2024. p. 34. | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/63948 | |
| Descripción | Os autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz. | en_US |
| Idioma | eng | en_US |
| Editor | Instituto de Tecnologia em Imunobiológicos | en_US |
| Derechos de autor | open access | en_US |
| Palabras clave en Portugués | Raiva | en_US |
| Palabras clave en Portugués | mRNA | en_US |
| Palabras clave en Portugués | Sistemas de entrega | en_US |
| Título | Expression of the Rabies Virus Glycoprotein in vitro through three mRNA Delivery Systems | en_US |
| Tipo del documento | Papers presented at events | en_US |
| DOI | 10.35259/isi.biomang.2024_63948 | |
| Resumen en Inglés | Introduction: Rabies is an infectious viral disease transmitted through the saliva of infected animals causing severe encephalitis and death. Its control has been a global challenge given the cost of prevention and treatment, especially in underdeveloped and emerging countries, where cases are endemic. Available vaccines have proven efficacy, but they present high production costs and biosafety risks. New technologies have proven to be effective, safer, and less costly for production. One of them is the use of mRNA vaccines. Objectives: This study aims to compare the expression of the rabies virus glycoprotein in three different mRNA delivery systems. For this study, a self-replicating mRNA will be used. Methodology: Plasmids were obtained through transformation and DNA extraction, as well as confirmation by restriction patterns, and subsequently, in vitro transcription was performed. Adherent BHK-21 cells were transfected with lipofectamine, lipid nanoparticles containing mRNA-RVGP or viral pseudoparticles. After 24 or 48h, the cells were fixed and submitted to indirect immunofluorescence assays (IF) by using specifc anti-RVGP antibody and Alexa Fluor conjugate to verify protein expression. Results: After preliminary tests, by immunofluorescence assays an expression of the rabies virus glycoprotein can be seen in cells treated with different delivery systems, at different intensities. The intensity also varies according to the incubation time in the analyzed period from 24h to 48h, however, in some delivery conditions fluorescence intensity is not dependent on the mRNA amount. Conclusion: The three delivery systems of the same tested mRNA proved to be effective, however, complementary tests will be necessary to quantitatively analyze protein production. In addition, new kinetic tests will be performed to assess how long the protein expression. | en_US |
| Afiliación | Instituto Butantan. São Paulo, SP, Brasil / Universidade de São Paulo. São Paulo, SP, Brasil. | en_US |
| Afiliación | Instituto Butantan. São Paulo, SP, Brasil / Universidade de São Paulo. São Paulo, SP, Brasil. | en_US |
| Afiliación | Instituto Butantan. São Paulo, SP, Brasil. | en_US |
| Afiliación | Instituto Butantan. São Paulo, SP, Brasil. | en_US |
| Afiliación | Instituto Butantan. São Paulo, SP, Brasil. | en_US |
| Afiliación | Instituto Butantan. São Paulo, SP, Brasil. | en_US |
| Palavras clave en Inglês | Rabies | en_US |
| Palavras clave en Inglês | mRNA | en_US |
| Palavras clave en Inglês | Delivery systems | en_US |
| DeCS | Raiva | en_US |
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