| Author | Ondei, Roberta | |
| Author | Dohanik, Caroline | |
| Author | Oliveira, Leandro Gonzaga de | |
| Author | Christensen, Dennis | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Fernandes, Ana Paula | |
| Author | Goulart, Gisele Assis Castro | |
| Access date | 2024-05-07T13:38:51Z | |
| Available date | 2024-05-07T13:38:51Z | |
| Document date | 2024 | |
| Citation | ONDEI, Roberta et al. EmulsivantTM 70 as a novel chemistry for oil-based vaccines: pre-clinical and safety studies in tetanus toxoid model. In: INTERNATIONAL SYMPOSIUM ON IMMUNOBIOLOGICAL, 8., 2024. Rio de Janeiro. Annals... Rio de Janeiro: Bio-Manguinhos, 2024. p. 36. | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/63958 | |
| Description | Os autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz. | en_US |
| Language | eng | en_US |
| Publisher | Instituto de Tecnologia em Imunobiológicos | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | EmulsivanteTM 70 | en_US |
| Subject in Portuguese | Segurança | en_US |
| Subject in Portuguese | Eficácia | en_US |
| Title | EmulsivantTM 70 as a novel chemistry for oil-based vaccines: pre-clinical and safety studies in tetanus toxoid model | en_US |
| Type | Papers presented at events | en_US |
| DOI | 10.35259/isi.biomang.2024_63958 | |
| Abstract | Introduction: Adjuvants are normally used to increase the effectiveness of vaccines, being oil-based emulsified adjuvants of great relevance for veterinary vaccines. In this context, EmulsivantTM 70, a novel ready-to-use water- in-oil (W/O) emulsion-based adjuvant, was evaluated for efficacy and safety in mouse model using tetanus toxoid antigen (TT). Objectives: To evaluate the safety and efficacy profile of EmulsivantTM 70 in pre-clinical studies using TT antigen and compare its performance to MontanideTM ISA 70, a commercial adjuvant product composed of mannitol monooleate mineral oil. Methodology: EmulsivantTM 70 and MontanideTM ISA 70 emulsions were prepared under standard procedure. The studies were carried out with female-specific pathogen-free mice (BALB/c, 6-8 weeks of age) in accordance with the UFMG Ethics Committee on Animal Experimentation (CEUA/UFMG no LW16/20). Animals were randomly assigned into 5 treatment groups (n=8/group): 1. phosphate buffered saline loaded with TT; 2. TT plus Alhydrogel® and CpG (positive control); 3. EmulsivantTM 70 emulsion without TT; 4. EmulsivantTM 70 emulsion loaded with TT; 5. MontanideTM ISA 70 emulsion loaded with TT. The 5 groups received the 1st immunization on day 0, the booster on day 21 and were euthanized on day 51. The isotype profile of IgG and renal and hepatic biomarkers were evaluated on days 0, 21, and 51. The IFN-γ production and integrity tissue at the application site were also evaluated at the end of the experiment. Results: EmulsivantTM 70 emulsions showed no difference in reactogenicity at the application site when compared to MontanideTM ISA 70 emulsion. There were no key changes in any evaluated biochemical markers. Both adjuvants induced higher titers of IgG and IgG1. However, immunization with EmulsivantTM 70 emulsion increased total IgG titers more rapidly. IFN-γ levels were increased without difference between the adjuvants. Conclusion: This pre-clinical study with EmulsivantTM 70 using TT as an antigen model provided evidence that this adjuvant is as safe and effective as the commercial adjuvant MontanideTM ISA 70 in mice but increased total IgG titers more quickly. | en_US |
| Affilliation | Croda Brazil. Campinas, SP, Brasil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Detechta. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Croda Denmark. Frederikssund, Dinamarca. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | en_US |
| Subject | EmulsivantTM 70 | en_US |
| Subject | Safety | en_US |
| Subject | Effectiveness | en_US |
| DeCS | Segurança | en_US |
| DeCS | Eficácia | en_US |
