| Author | Fiuza, Bianca Sampaio Dotto | |
| Author | Andrade, Candace Machado de | |
| Author | Meirelles, Pedro Milet | |
| Author | Silva, Jorley Santos da | |
| Author | Silva, Milca de Jesus | |
| Author | Santana, Cinthia Vila Nova | |
| Author | Pinheiro, Gabriela Pimentel | |
| Author | Mpairwe, Harriet | |
| Author | Cooper, Philip | |
| Author | Brooks, Collin | |
| Author | Pembrey, Lucy | |
| Author | Taylor, Steven | |
| Author | Douwes, Jeroen | |
| Author | Cruz, Álvaro A. | |
| Author | Barreto, Mauricio L. | |
| Author | Pearce, Neil | |
| Author | Figueiredo, Camila A. V. | |
| Access date | 2024-05-17T17:56:25Z | |
| Available date | 2024-05-17T17:56:25Z | |
| Document date | 2024 | |
| Citation | FIUZA, Bianca Sampaio Dotto et al. Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma. Journal of Allergy and Clinical Immunology, v. 3, n. 2, 1-10, 2024. | en_US |
| ISSN | 1097-6825 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64126 | |
| Sponsorship | European Research Council under the European Union’s, Seventh Framework Programme.
Coordenação de Aperfeiçãooamento de Pessoal de Nível Superior (CAPES).
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | en_US |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Asma | en_US |
| Subject in Portuguese | Microbioma | en_US |
| Subject in Portuguese | Banco | en_US |
| Subject in Portuguese | Microbiota intestinal | en_US |
| Title | Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma | en_US |
| Type | Article | en_US |
| DOI | 10.1016/j.jacig.2024.100242 | |
| Abstract | Background: Asthma is a complex disease and a severe global public health problem resulting from interactions between genetic background and environmental exposures. It has been suggested that gut microbiota may be related to asthma development; however, such relationships needs further investigation. Objective: This study aimed to characterize the gut microbiota as well as the nasal lavage cytokine profile of asthmatic and nonasthmatic individuals.
Methods: Stool and nasal lavage samples were collected from 29 children and adolescents with type 2 asthma and 28 children without asthma in Brazil. Amplicon sequencing of the stool bacterial V4 region of the 16S rRNA gene was performed using Illumina MiSeq. Microbiota analysis was performed by QIIME 2 and PICRUSt2. Type 2 asthma phenotype was characterized by high sputum eosinophil counts and positive skin prick tests for house dust mite, cockroach, and/or cat or dog dander. The nasal immune marker profile was assessed using a customized multiplex panel. Results: Stool microbiota differed significantly between asthmatic and nonasthmatic participants (P = .001). Bacteroides was more abundant in participants with asthma (P < .05), while Prevotella was more abundant in nonasthmatic individuals (P < .05). In people with asthma, the relative abundance of Bacteroides correlated with IL-4 concentration in nasal lavage samples. Inference of microbiota functional capacity identified differential fatty acid biosynthesis in asthmatic compared to nonasthmatic subjects. Conclusion: The stool microbiota differed between asthmatic and nonasthmatic young people in Brazil. Asthma was associated with higher Bacteroides levels, which correlated with nasal IL-4 concentration. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Instituto de Biologia, Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciencia e Tecnologia em Estudos Interdisciplinares e Transdisciplinares em Ecologia e Evolucao (IN-TREE). Salvador, BA, Brasil. | en_US |
| Affilliation | Instituto de Biologia, Universidade Federal da Bahia. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundacao ProAR Salvador. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundacao ProAR Salvador. Salvador, BA, Brasil. | en_US |
| Affilliation | MRC/UVRI and LSHTM Uganda Research Unit. Entebbe, Uganda. | en_US |
| Affilliation | Fundacion Ecuatoriana Para Investigacion en Salud. Quito, Ecuador / Universidad Internacional del Ecuador. Quito, Ecuador / St George’s University of London. United Kingdom. | en_US |
| Affilliation | Centre for Public Health Research, Massey University, Wellington, New Zealand. | en_US |
| Affilliation | London School of Hygiene and Tropical Medicine. London, United Kingdom. | en_US |
| Affilliation | College of Medicine and Public Health. Flinders University. Adelaide, Austrália. | en_US |
| Affilliation | Centre for Public Health Research, Massey University, Wellington, New Zealand. | en_US |
| Affilliation | Fundacao ProAR Salvador. Salvador, BA, Brasil / Faculdade de Medicina, Universidade Federal da Bahia. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Centro de Integração de Dados e Conhecimentos para Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Centre for Public Health Research, Massey University, Wellington, New Zealand / London School of Hygiene and Tropical Medicine. London, United Kingdom. | en_US |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | en_US |
| Subject | Asthma | en_US |
| Subject | Microbiome | en_US |
| Subject | Stool | en_US |
| Subject | Gut microbiota | en_US |
| DeCS | Asma | en_US |
| DeCS | Micobioma | en_US |
| DeCS | Microbioma gastrointestinal | en_US |
