| Author | Sarno, Alice | |
| Author | Leite, Avelina | |
| Author | Augusto, Carlos | |
| Author | Muller, Igor | |
| Author | Ângelis, Luanna de | |
| Author | Pimentel, Lilian | |
| Author | Queiroz, Adriano | |
| Author | Arruda, Sergio | |
| Access date | 2024-05-21T14:26:04Z | |
| Available date | 2024-05-21T14:26:04Z | |
| Document date | 2024 | |
| Citation | SARNO, Alice et al. Impaired macrophage and memory T-cell responses to Bacillus Calmette-Guerin nonpolar lipid extract. Frontiers in Immunology, v. 14, p. 1-9, 2024. | en_US |
| ISSN | 1664-3224 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64161 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). | en_US |
| Language | eng | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Bacilo Calmette-Guerin | en_US |
| Subject in Portuguese | Mycobacterium tuberculose | en_US |
| Subject in Portuguese | Extratos lipídicos não polares | en_US |
| Subject in Portuguese | Expressão gênica de macrófagos | en_US |
| Subject in Portuguese | Respostas de células T de memória | en_US |
| Title | Impaired macrophage and memory T-cell responses to Bacillus Calmette-Guerin nonpolar lipid extract | en_US |
| Type | Article | en_US |
| DOI | 10.3389/fimmu.2023.1263352 | |
| Abstract | Introduction: The attenuation of BCG has led to the loss of not only immunogenic proteins but also lipid antigens. Methods: Thus, we compared the macrophage and T-cell responses to nonpolar lipid extracts harvested from BCG and Mycobacterium tuberculosis (Mtb) to better understand the role of BCG lipids in the already known diminished responses of the vaccine strain. Results: Relative to Mtb, nonpolar lipid extract from BCG presented a reduced capacity to trigger the expression of the genes encoding TNF, IL-1b, IL-6 and IL-10 in RAW 264.7 macrophages. Immunophenotyping of PBMCs isolated from healthy individuals revealed that lipids from both BCG and Mtb were able to induce an increased frequency of CD4+ and CD8+ T cells, but only the lipid extract from Mtb enhanced the frequency of CD4-CD8-double-negative, γσ+, CD4+HLA-DR+, and γσ+HLA-DR+ T cells relative to the nonstimulated control. Interestingly, only the Mtb lipid extract was able to increase the frequency of CD4+ memory (CD45RO+) T cells, whereas the BCG lipid extract induced a diminished frequency of CD4+ central memory (CD45RO+CCR7-) T cells after 48 h of culture compared to Mtb. Discussion: These findings show that the nonpolar lipids of the BCG bacilli presented diminished ability to trigger both proinflammatory and memory responses and suggest a potential use of Mtb lipids as adjuvants to increase the BCG vaccine efficacy. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoepidemiologia. Recife, PE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoepidemiologia. Recife, PE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida, Salvador, Brasil. | en_US |
| Subject | Bacillus Calmette-Guerin | en_US |
| Subject | Mycobacterium tuberculosis | en_US |
| Subject | Nonpolar lipid extracts | en_US |
| Subject | Macrophage gene expression | en_US |
| Subject | Memory T-cell responses | en_US |
| DeCS | Tuberculose | en_US |
| DeCS | Expressão gênica | en_US |
| DeCS | Macrófagos | en_US |
| DeCS | Linfócitos T | en_US |
