| Author | Coelho, Ivo C. B. | |
| Author | Haguinet, François | |
| Author | Colares, Jeová Keny B. | |
| Author | Coelho, Zirlane C. B. | |
| Author | Araújo, Fernanda M. C. | |
| Author | Schwarcz, Waleska Dias | |
| Author | Duarte, Ana Claudia | |
| Author | Borges, Beatriz | |
| Author | Minguet, Catherine | |
| Author | Guignard, Adrienne | |
| Access date | 2024-05-23T13:59:20Z | |
| Available date | 2024-05-23T13:59:20Z | |
| Document date | 2020 | |
| Citation | COELHO, Ivo C. B. et al. Dengue Infection in Children in Fortaleza, Brazil: A 3-Year School-Based Prospective Cohort Study. The American Journal of Tropical Medicine and Hygiene, v. 103, p. 100-111, Jul. 2020. | en_US |
| ISSN | 0002-9637 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64202 | |
| Language | eng | en_US |
| Publisher | American Society of Tropical Medicine and Hygiene | en_US |
| Rights | open access | en_US |
| Title | Dengue infection in children in Fortaleza, Brazil: A 3-year school-based prospective cohort study | en_US |
| Type | Article | en_US |
| DOI | 10.4269/ajtmh.19-0521 | |
| Abstract | Dengue is endemic in Brazil. The dengue surveillance system’s reliance on passive reporting may underestimate disease incidence and cannot detect asymptomatic/pauci-symptomatic cases. In this 3-year prospective cohort study (NCT01391819) in 5- to 13-year-old children from nine schools in Fortaleza (N = 2,117), we assessed dengue virus (DENV) infection seroprevalence by IgG indirect ELISA at yearly visits and disease incidence through active and enhanced passive surveillance. Real-time quantitative polymerase chain reaction (RT-qPCR) and DENV IgM/IgG capture ELISA were used for diagnosis. We further characterized confirmed and probable cases with a plaque reduction neutralization test. At enrollment, 54.1% (95% CI: 46.6, 61.4) of children were DENV IgG positive. The annual incidence of laboratory-confirmed symptomatic dengue cases was 11.0 (95% CI: 7.3, 14.7), 18.1 (10.4, 25.7), and 10.2 (0.7, 19.7), and of laboratory-confirmed or probable dengue cases with neutralizing antibody profile evocative of dengue exposure was 13.2 (6.6, 19.9), 18.7 (5.3, 32.2), and 8.4 (2.4, 19.2) per 1,000 child-years in 2012, 2013, and 2014, respectively. By RTqPCR, we identified 14 DENV-4 cases in 2012–2013 and seven DENV-1 cases in 2014. During the course of the study, 32.8% of dengue-naive children experienced a primary infection. Primary inapparent dengue infection was detected in 20.3% (95% CI: 13.6, 29.1) of dengue-naive children in 2012, 8.7% (6.9, 10.9) in 2013, and 5.1% (4.4, 6.0) in 2014. Our results confirmed the high dengue endemicity in Fortaleza, with active and enhanced passive surveillance detecting three to five times more cases than the National System of Disease Notification. | en_US |
| Affilliation | Universidade Federal do Ceará. Fortaleza, CE, Brasil. | en_US |
| Affilliation | GlaxoSmithKline, Wavre, Belgium. | en_US |
| Affilliation | Secretaria de Saúde do Estado do Ceará. Hospital São José de Doenças Infecciosas. Fortaleza, CE, Brasil / Universidade de Fortaleza. Programa de Pós-Graduação em Ciências Médicas. Fortaleza, CE, Brasil. | en_US |
| Affilliation | Universidade Federal do Ceará. Fortaleza, CE, Brasil. | en_US |
| Affilliation | Laboratório Central de Saúde Publica do Ceará. Fortaleza, CE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | GSK, Rixensart, Belgium. | en_US |
| Affilliation | GlaxoSmithKline, Wavre, Belgium. | en_US |
| Subject | Dengue Virus | en_US |
| Subject | Brazil | en_US |
| Subject | Neutralization Tests | en_US |
| Subject | Antibodies | en_US |
| Subject | Children | en_US |
