| Author | Carvalho, Augusto M. | |
| Author | Guimarães, Luiz H. | |
| Author | Costa, Rúbia | |
| Author | Carvalho, Lucas P. | |
| Author | Arruda, Sérgio | |
| Author | Carvalho, Edgar M. | |
| Access date | 2024-06-05T13:17:28Z | |
| Available date | 2024-06-05T13:17:28Z | |
| Document date | 2022 | |
| Citation | CARVALHO, Augusto M. et al. Impaired TH1 immune response is associated with disease severity and treatment outcome in cutaneous leishmaniasis patients. In: CONGRESSO WORLD LEISH7, 7, 2022., Colômbia. Anais eletrônicos [...]. Colômbia: CIDEPRO, 23 ago. 2022. p. 1-15. | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64307 | |
| Language | eng | en_US |
| Publisher | CIDEPRO | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Leishmaniose cutânea | en_US |
| Subject in Portuguese | Teste cutâneo de leishmania | en_US |
| Subject in Portuguese | Resposta TH1 | en_US |
| Subject in Portuguese | Inflamação | en_US |
| Subject in Portuguese | Imunopatologia | en_US |
| Title | Impaired TH1 immune response is associated with disease severity and treatment outcome in cutaneous leishmaniasis patients | en_US |
| Type | Papers presented at events | en_US |
| Abstract | Control of Leishmania infection relies on cell-mediated immune response, as IFN-γ induced activation of macrophages is known to be the main mechanism of parasite killing within these cells. Peripheral blood mononuclear cells (PBMC) from patients with cutaneous leishmaniasis (CL) caused by Leishmania braziliensis infection, secrete high levels of IFN-γ and TNF that contributes for parasite control but also is associated to pathology. Moreover, CL patients presents a positive delayed-type hypersensitivity reaction to Leishmania antigens as known Leishmania skin test. Interestingly, around 4% of patients with CL present a negative LST. Here, we compare the clinical presentation, response to therapy, and immune response of CL patients who are LST negative (n =72) with those who are LST positive (n =72). Patients with negative LST had significantly larger ulcers than LST positive group. Furthermore, number of lesions was higher in LST negative subjects compared to LST positive patients. While cure at day 90 post therapy was 70% in the control group, it was 43% in the LST-negative patients. In addition, ulcers healed slower in the LST-negative patients than in the LST-positive. To evaluate immune response, cytokines production in lesion biopsy supernatants was assessed by ELISA. LST-negative patients had a poor Th1 response (IFN-γ and TNF) but levels of IL-1β, IL-6, IL-17, granzyme B, and perforin were similar to the LST-positive group. In this study, we observed that in LST-negative patients, impaired Th1 response is associated with therapeutic failure. Moreover, high production of innate related inflammatory cytokines, and cytotoxic molecules such as granzyme B and perforin contributes to immunopathology. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Subject | Cutaneous leishmaniasis | en_US |
| Subject | Leishmania skin test | en_US |
| Subject | TH1 response | en_US |
| Subject | Inflammation | en_US |
| Subject | Immunopathology | en_US |
| DeCS | Leishmaniose cutânea | en_US |
| DeCS | Inflamação | en_US |
