| Author | Iani, Felipe Campos de Melo | |
| Author | Campos, Gabriel Montenegro de | |
| Author | Adelino, Talita Emile Ribeiro | |
| Author | Silva, Anielly Sarana da | |
| Author | Kashima, Simone | |
| Author | Alcantara, Luiz Carlos Junior | |
| Author | Sampaio, Sandra Coccuzzo | |
| Author | Giovanetti, Marta | |
| Author | Elias, Maria Carolina | |
| Author | Slavov, Svetoslav Nanev | |
| Access date | 2024-06-05T16:34:52Z | |
| Available date | 2024-06-05T16:34:52Z | |
| Document date | 2024 | |
| Citation | IANI, Felipe Campos de Melo et al. Metagenomic Analysis for Diagnosis of Hemorrhagic Fever in Minas Gerais, Brazil. Microorganisms, v. 12, n. 4, p. 769, 2024. doi.org/10.3390/microorganisms12040769. | en_US |
| ISSN | 2076-2607 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64327 | |
| Language | eng | en_US |
| Publisher | MPDI | en_US |
| Rights | open access | en_US |
| Title | Metagenomic Analysis for Diagnosis of Hemorrhagic Fever in Minas Gerais, Brazil. | en_US |
| Type | Article | en_US |
| Abstract | Viral hemorrhagic fever poses a significant public health challenge due to its severe clinical presentation and high mortality rate. The diagnostic process is hindered by similarity of symptoms across different diseases and the broad spectrum of pathogens that can cause hemorrhagic fever. In this study, we applied viral metagenomic analysis to 43 serum samples collected by the Public Health Laboratory (Fundação Ezequiel Dias, FUNED) in Minas Gerais State, Brazil, from patients diagnosed with hemorrhagic fever who had tested negative for the standard local hemorrhagic disease testing panel. This panel includes tests for Dengue virus (DENV) IgM, Zika virus IgM, Chikungunya virus IgM, yellow fever IgM, Hantavirus IgM, Rickettsia rickettsii IgM/IgG, and Leptospira interrogans IgM, in addition to respective molecular tests for these infectious agents. The samples were grouped into 18 pools according to geographic origin and analyzed through next-generation sequencing on the NextSeq 2000 platform. Bioinformatic analysis revealed a prevalent occurrence of commensal viruses across all pools, but, notably, a significant number of reads corresponding to the DENV serotype 2 were identified in one specific pool. Further verification via real-time PCR confirmed the presence of DENV-2 RNA in an index case involving an oncology patient with hemorrhagic fever who had initially tested negative for anti-DENV IgM antibodies, thereby excluding this sample from initial molecular testing. The complete DENV-2 genome isolated from this patient was taxonomically classified within the cosmopolitan genotype that was recently introduced into Brazil. These findings highlight the critical role of considering the patient's clinical condition when deciding upon the most appropriate testing procedures. Additionally, this study showcases the potential of viral metagenomics in pinpointing the viral agents behind hemorrhagic diseases. Future research is needed to assess the practicality of incorporating metagenomics into standard viral diagnostic protocols. | en_US |
| Affilliation | Laboratory of Virology. Ezequiel Dias Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Blood Center of Riberirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Laboratory of Virology. Ezequiel Dias Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Laboratory of Virology. Ezequiel Dias Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Blood Center of Riberirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Climate Amplified Diseases and Epidemic. Brasilia, DF, Brazil. | en_US |
| Affilliation | Laboratory of Cell Cycle. Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
| Affilliation | Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Climate Amplified Diseases and Epidemic. Brasilia, DF, Brazil/Department of Science and Technology for Humans and the Environment. University of Campus Bio-Medico di Roma. Rome, Italy/Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Arbovírus e Vírus Hemorrágicos. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Laboratory of Cell Cycle. Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
| Affilliation | Laboratory of Cell Cycle. Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
| Subject | Brazil | en_US |
| Subject | hemorrhagic fever | en_US |
| Subject | next-generation sequencing | en_US |
| Subject | viral diagnosis | en_US |
| Subject | viral metagenomics | en_US |
