| Author | Mateu, G. P. | |
| Author | Marchevsky, R. S. | |
| Author | Liprandi, F. | |
| Author | Bonaldo, M. C. | |
| Author | Coutinho, E. S. F. | |
| Author | Dieudonné, M. | |
| Author | Caride, E. | |
| Author | Jabor, A. V. | |
| Author | Freire, M. S. | |
| Author | Galler, R. | |
| Access date | 2024-06-13T12:15:38Z | |
| Available date | 2024-06-13T12:15:38Z | |
| Document date | 2007 | |
| Citation | MATEU, G. P. et al. Construction and biological properties of yellow fever 17D/dengue type 1 recombinant virus. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 101, n. 3, p. 289-298, Mar. 2007. | en_US |
| ISSN | 0035-9203 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64476 | |
| Language | eng | en_US |
| Publisher | Oxford University Press | en_US |
| Rights | restricted access | |
| Title | Construction and biological properties of yellow fever 17D/dengue type 1 recombinant virus | en_US |
| Type | Article | |
| DOI | 10.1016/j.trstmh.2006.08.006 | |
| Abstract | Dengue virus, a mosquito-borne flavivirus, is one of the most formidable public health threats in tropical and subtropical regions. As yet, there is no licensed vaccine to protect against the disease. A chimeric yellow fever (YF) 17D/dengue (DEN) type 1 virus was constructed by replacing the pre-membrane and envelope genes of YF 17D virus with those from DEN 1 VeMir95 virus, a Venezuelan isolate. The chimeric YF 17D/DEN 1 VeMir95 virus was regenerated from full-length infectious clones stably propagated in Escherichia coli by transfection of Vero cells with in vitro transcribed RNA. The chimeric virus proliferated efficiently in Vero cells (∼6.6 log10 plaque-forming units/ml). The chimeric virus was not neurovirulent to 3- week-old Swiss Webster mice inoculated by the intracerebral route, in contrast to the YF 17DD vaccine strain that was lethal for 90% of the mice. The YF 17D/DEN 1 virus at Passage 6 was more attenuated for rhesus monkeys than the YF 17DD commercial vaccine after intracerebral inoculation according to the standard neurovirulence test. This virus is a potential candidate to be included in a tetravalent DEN vaccine formulation. The availability of the cloned cDNA allows further structure/function studies on the viral envelope. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologıa Molecular. Rio de Janeiro, RJ, Brasil / Instituto Venezolano de Investigaciones Científicas. Caracas, Venezuela. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Instituto Venezolano de Investigaciones Científicas. Caracas, Venezuela. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologıa Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Instituto Venezolano de Investigaciones Científicas. Caracas, Venezuela. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologıa Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Yellow fever | en_US |
| Subject | Dengue | en_US |
| Subject | Envelope gene | en_US |
| Subject | Attenuation | en_US |
| Subject | Vaccine | en_US |
| Embargo date | 2030-12-31 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
