| Author | Jacob-Nascimento, L. C. | |
| Author | Portilho, M. M. | |
| Author | Moreira, P. S. S. | |
| Author | Anjos, R. O. | |
| Author | Reis, M. G. | |
| Author | Ribeiro, G. S. | |
| Access date | 2024-06-18T16:31:09Z | |
| Available date | 2024-06-18T16:31:09Z | |
| Document date | 2022 | |
| Citation | JACOB-NASCIMENTO, L. C. et al. Detection of Chikungunya virus RNA in oral fluid and urine: an alternative approach to diagnosis? In: CONGRESSO DE MEDICINA TROPICAL DA AMÉRICA LATINA (MEDTROP), 57, 2022, Belém, PA. Anais eletrônicos [...] Belém, PA: SBMT, 13 a 16 de novembro de 2022. p.1. | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64545 | |
| Language | eng | en_US |
| Publisher | Sociedade Brasileira de Medicina Tropical | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Chikungunya | en_US |
| Subject in Portuguese | qRT-PCR | en_US |
| Subject in Portuguese | Diagnóstico molecular | en_US |
| Subject in Portuguese | Fluido oral de urina | en_US |
| Title | Detection of Chikungunya virus RNA in oral fluid and urine: an alternative approach to diagnosis? | en_US |
| Alternative title | Detecção do RNA do vírus Chikungunya em fluido oral e urina: uma abordagem alternativa para o diagnóstico? | en_US |
| Type | Papers presented at events | en_US |
| Abstract | Objetivo(s): To evaluate whether oral fluid (OF) and urine can be used as alternative samples to serum to diagnose chikungunya virus (CHIKV) infection by qRT-PCR. Material e Métodos: Serum, OF (collected with a rayon-tipped swab in the sublingual region), and urine samples used in the evaluation were obtained from patients enrolled in a prospective surveillance study to detect arbovirus infection among cases of acute febrile illness (≤7 days of symptom onset). The studied samples belonged to three groups of patients with laboratory evidence of chikungunya: patients with qRT-PCR positive for CHIKV in acute-phase sera (n=19); with CHIKV IgM seroconversion between
paired sera (n=12); and with CHIKV IgM detection in acute-phase sera (n=20). The last two groups were negative for CHIKV by qRT-PCR in acute-phase serum. As controls, we used sera, OF, and urine from patients with other acute febrile illnesses (n=28) and from patients with RT-PCR-confirmed dengue (n=16). All samples underwent RNA extraction (Maxwell®, Promega, Madison, USA), followed by CHIKV qRT-PCR (CDC-TRIOPLEX). CHIKV IgM antibodies were investigated using IgM ELISA kits (InBios, Seattle, USA). Resultados e Conclusão: Of the 19 patients with qRT-PCR positive for CHIKV in sera, 9 (47.4%) and one (5.2%) were also positive by qRT-PCR in OF and urine, respectively. Of the 12 patients with
evidence of CHIKV infection solely based on CHIKV IgM seroconversion, 2 (16.7%) were positive for CHIKV by qRT-PCR in OF, and none in urine. None of the 20 patients belonging to the group with CHIKV IgM antibodies in acute-phase sera were positive for CHIKV by qRT-PCR in OF and urine. None of the samples from the control groups were positive (specificity: 100%).Although the CHIKV qRT-PCR in OF was positive in some patients with CHIKV IgM seroconversion who were negative by qRT-PCR in sera, the qRT-PCR sensitivity in OF was about 50% among patients who had a positive CHIKV qRT-PCR in serum. Thus, while OF may be useful as an alternative non-invasive specimen to diagnose acute
chikungunya, a negative result cannot rule out an infection. The frequency at which acute-phase urine showed detectable levels of CHIKV RNA in patients with detectable CHIKV RNA in sera was too low to suggest the use of urine as an alternative acute-phase sample. Further research is needed to evaluate whether OF and urine collected later in the disease course may be helpful in diagnosing chikungunya. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Yale School of Public Health. Yale University. New Haven, Estados Unidos. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil. | en_US |
| Subject | Chikungunya | en_US |
| Subject | qRT-PCR | en_US |
| Subject | Molecular diagnosis | en_US |
| Subject | Urine oral fluid | en_US |
| DeCS | Vírus chikungunya | en_US |
| DeCS | Técnicas de diagnóstico molecular | en_US |
| DeCS | Urina | en_US |
